Toxic and genotoxic effect of 1-(4-chlorobenzyl)-3-chloro-4-(trifluoromethyl-phenylamino)-1h-pyrol-2,5-dione in the test system Drosophila melanogaster Mg. (Diptera: Drosophilidae)

  • O. V. Protsenko
  • O. A. Dudka
  • S. V. Demydov
  • I. A. Kozeretska
DOI: https://doi.org/10.7124/FEEO.v21.809

Abstract

Aim. To date, the problem of cancer treatment is very acute particularly in countries where the population faced significant man-made catastrophes. It was shown that 1-(4-chlorobenzyl)-3-chloro-4-(trifluoromethyl-phenylamino)-1H-pyrol-2,5-dione can be used as a cure for cancer. Studied compound shows remarkable antiproliferative effect on the culture of transformed and cancer cells. However, as any drug this compound is a potential xenobiotics and requires pre-test for genotoxicity. Methods. To study the toxicity of chemical determined it was analyzed survival, development time and fecundity of flies. We evaluated the genotoxicity of the potential drug with the test for DNA damage repair on the model organism – Drosophila melanogaster. For study we used the strain mei-9a mei-41D5 / FM7c; mwh, y for the detection of repairable DNA damage in somatic cells. The tested compound was added to culture medium in amount of 1 mg per 4 ml of the medium. Results. As a result, we did not observe any toxic or genotoxic effects for D. melanogaster in concentration 0.2 mg/ml. Conclusions. It was shown the absents of the ability compound, cause toxic and genotoxic effects at the model organism Drosophila melanogaster.
Keywords: genotoxic, anticancer drugs, Drosophila melanogaster.

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