Locomotor activity of Drosophila melanogaster larvae with altered function of gene swiss cheese

  • N. P. Matiytsiv
  • O. I. Trush
  • O. M. Gudyma
  • Ya. I. Chernyk
DOI: https://doi.org/10.7124/FEEO.v21.803

Abstract

Aim. Gene swiss cheese (sws) in Drosophila melanogaster is ortholog of human gene Neuropathy Target Esterase (NTE). Point mutation in this gene lead to many neurodegenerative disorders, such as Spastic Paraplegia. The aim of this study was to determine whether the change of gene sws function influence in Drosophila larvae motor behavior. Methods. We used two mutant lines: sws1 and sws76-15; transgenic lines for UAS-Gal4 controlled expression of SWS in moroneurons. Locomotor assay was performed in third-instar larvae. Results. We have found that larvae sws76-15 and sws1 showed significant decrease of motor behavior in comparison with wild type: sws1 has 35.4 %, and sws76-15 24.1 % difference with Oregon R. Functional knockout of sws in motoneuron showed decrease of motor activity as well – 16.5 % less than control. Оverexpression sequences of complete SWS protein in motoneurons causes significant increment in motor activity about 43.7 %. Esterase domen and three nucleotidbinding domains overexpression in larvae motoneurons had no differences compared to control. Conclusions. For the first time was shown a critical role of gene sws for larvae motor activity in D. melanogaster. Flies with altered sws function are good model of spastic paraplegia study.

Keywords: Drosophila melanogaster, sws/NTE, neuropathy, locomotor behavior.

References

Mühlig-Versen M., da Cruz A.B., Tschäpe J.A., Moser M., Büttner R., Athenstaedt K., Glynn P., Kretzschmar D. Loss of Swiss cheese/neuropathy target esterase activity causes disruption of phosphatidylcholine homeostasis and neuronal and glial death in adult Drosophila. J Neurosci. 2005. V. 25. P. 2865-2873. doi: 10.1523/JNEUROSCI.5097-04.2005

Moser M., Stempfl T., Li Y., Glynn P., Büttner R., Kretzschmar D. Cloning and expression of the murine sws/NTE gene. Mech Dev. 2000. V. 90. P. 279-282. doi: 10.1016/S0925-4773(99)00239-7

Akassoglou K., Malester B., Xu J., Tessarollo L., Rosenbluth J., Chao M.V. Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration. Proc Natl Acad Sci USA. 2004. V. 101. P. 5075-5080. doi: 10.1073/pnas.0401030101

Rainier S., Bui M., Mark E., Thomas D., Tokarz D., Ming L., Delaney C., Richardson R.J., Albers J.W., Matsunami N., Stevens J., Coon H., Leppert M., Fink J.K. Neuropathy Target Esterase Gene Mutations Cause Motor Neuron Disease. The American Journal of Human Genetics. 2008. V. 82. P. 780-785. doi: 10.1016/j.ajhg.2007.12.018

Fink J.K. Hereditary spastic paraplegia. Curr. Neurol. Neurosci. Rep. 2006. V. 6. P. 65-76. doi: 10.1007/s11910-996-0011-1

Johnson M. The delayed neurotoxic action of some organophosphorus compounds. Identification of the phosphorylation site as an esterase. Biochem. J. 1969. V. 114. P. 711-717. doi: 10.1042/bj1140711

Glynn P. Mechanism for organophosphate-induced delayed neuropathy. Toxicology Letters. 2006. V. 162. P. 94-97. doi: 10.1016/j.toxlet.2005.10.012

Shcherbata G.R., Matiytsiv, N.P., Chernik, Ya.I., Jatsenko A.S., Radysh V.V., Kucherenko M.M., Maksimiv D.V. Genetic analysis of X-chromosome Drosophila melanogaster neurodegenerative mutants induced by ethylmethanesulphonate and nitrosoethylurea. Genetika. 2004. V. 40. P. 1286-1292. doi: 10.1023/B:RUGE.0000041388.04203.1b

Kretzschmar D., Hasan S., Sharma S., Heisenberg M., Benzer S. The swiss cheese mutant cause glial hyperwrapping and brain degeneration in Drosophila. J. Neurosci. 1997. V. 17. P. 7425-7432. doi: 10.1523/JNEUROSCI.17-19-07425.1997

Mohyliak I., Matiytsiv N., Chernyk Ya. Vplyv tkanynno-spetsyfichnoho funktsional'noho inhibuvannia hena sws na formuvannia struktury skladnoho oka Drosophila melanogaster. Visnyk L'vivs'koho universytetu. Seriia biolohichna. 2013. Iss. 62. P. 117-122. [in Ukrainian]