Genomic biotechnology of assessment and selection of linseed breeding material

  • V. A. Lemesh
  • M. V. Bogdanova
  • E. L. Andronyk
  • I. A. Golub


Aim. The aim of this study was to develop of genomic biotechnology for the assessment and selection of the linssed breeding material by a complex of genes controlling the ratio of fatty acids in seeds oil to the creation of a new linseed variety. Methods. Breeding studies were combined with molecular-genetic studies. Results. We developed the genomic biotechnology to detect the mutant alleles of linseed fad3A and fad3B genes responsible for reduced α-linolenic acid levels in linseed oil. Using this biotechnology, it was possible to classify plants as homozygous mutant, homozygous wild type, or heterozygous at fad3A and fad3B loci, that can be used to breed new linseed varieties of food or industrial quality. Conclusions. By results of 3-year molecular-genetic and breeding studies the variety "Dar" was created with using the developed genomic biotechnology of an assessment and selection of linseed breeding material by a complex of the genes controlling the ratio of fatty acids in seeds oil.
Keywords: linseed (Linum usitatissimum L.), α-linolenic acid, fatty acid desaturase, fad3 genes.


Westcott N.D., Muir N.D. Chemical studies on the constituents of Linum spp. flax, the genus Linum. New York, 2003. P. 55-73.

Cullis C.A. Flax. Genome mapping and molecular breeding in plants / C. Kole. V. 2. Springer. Berlin, 2007. P. 275-295. doi: 10.1007/978-3-540-34388-2_8

Green A.G. A mutant genotype of flax (Linum usitatissimum L.) containing very low levels of linolenic acid in its seed oil. Can. J. Plant Sci. 1986. V. 66(3). P. 499-503. doi: 10.4141/cjps86-068

Rowland G.G. An EMS-induced low-linolenic-acid mutant in McGregor flax (Linum usitatissimum L.). J. Plant Sci. 1991. V. 71(2). P. 393-396. doi: 10.4141/cjps91-054

Dribnenki J.C.P., McEachern S.F., Chen Y., Green A.G., Rashid K.Y. 2149 Solin (low linolenic flax). Can. J. Plant Sci. 2007. V. 87(2). P. 297-299. doi: 10.4141/P05-082

Dribnenki J.C.P., Green A.G. Linola '947' low linolenic acid flax. Can. J. Plant Sci. 1995. V. 75(1). P.201-202. doi: 10.4141/cjps95-036

Vrinten P., Hu Z., Munchinsky M.-A., Rowland G., Qiu X. Two fad3 desaturase genes control the level of linolenic acid in flax seed. Plant Physiol. 2005. V. 139(1). P. 79-87. doi: 10.1104/pp.105.064451