Гетерологическая экспрессия Δ9-ацил-липидной десатуразы цианобактерии в орхидее Dendrobium linguella Rchb. F.
Aims. Heterologous expression of desaturases in plants may cause change in fatty acid spectrum and improve their cold resistance. Methods. Transgenic plants were selected and analyzed after Agrobacterium-mediated genetic transformation. Results. Protocols of D. linguella in vitro cultivation, genetic transformation and plant regeneration were developed. Transgenic plants of D. linguella carrying desС::licBM3 hybrid gene were selected and analyzed. Conclusions. D. linguella plants with high level of transgene expression showed change in fatty acid spectrum toward linolenic acid accumulation.
Key words: desaturase, transgenic plants, Dendrobium linguella.