Организация crt-кластеров штаммов из Streptomyces griseus группы

Л. В. Полищук, В. В. Лукьянчук

Анотація


Aim. The purpose of this study was to reveal similarities and differences in structures of crt-clusters of some strains from the Streptomyces griseus group. Methods. Resources of the server NCBI (programs BLAST: blast and bl2seq and database Genome (Chromosome) were used for analysis in silico of crt-cluster organizations of 17 strains from the S. griseus clade. Results. Search in silico in the database Genome (Chromosome) identified 45 strains that have been selected according to the degree of identity of the primary structures of their 16S rRNA gene sequences rrn1-gene necessary for S. griseus group members. The genomes of these 17 (37.7 %) strains had crt-clusters. 16 of them consisted of two convergent operons that combined 7 crt-genes. It showed the identity above 73 % of greater homologous fragments of crt-cluster (overlap 90 %). Only the crt-clusters of 15 strains were theoretically capable to synthesize carotenoids because crt-clusters of two strains (Streptomyces sp. Mg1, S. subrutilus 10-1-1) were with mutated crtE-genes and one cluster from the S. pristinaespiralis HCCB 10218 genome had no the crtE-gene. Conclusions. It was found the same scheme of crt-clusters organization in 17 studied strains from the S. griseus clade: they consisted of two convergent operons, which were mostly combined from 7 crt-genes (except was the strain S. pristinaespiralis HCCB 10218, whose cluster concluded of 6 genes).

Keywords: crt-cluster, organization of cluster, strain, genome, search in silico.


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