Цитогенетичне дослідження в клітинах кісткового мозку щурів – нащадків першого покоління батьків, які зазнали впливу інкорпорованого 131І
Aim. Among the problems that arose after the accident at the Chernobyl nuclear power plant belongs to the most important problem of the transmission effect of genomic instability through irradiated gametes parents first generation of their descendants. Determine the frequency of chromosomal aberrations in bone marrow cells of the offspring of rats that were exposed to the radioisotope 131I. Methods. Cytogenetic analysis of bone marrow cells in the offspring of the first generation of irradiated both parents exposed the females and intact males and irradiated malesan dintact females. Results. The results of experimental studies have shown that in the descendants of irradiated 131I females, the frequency of chromosome aberrations in bone marrow cells exceeds the control level (4.83 ± 0.59 % experience, 0.83 ± 0.36 % control). In offspring of exposed parents detected chromosomal abnormalities in bone marrow cells. The frequency of aberrant metaphases was significantly higher than the control level (4.16 ± 0.54 % and 0.83 ± 0.36). Cytogenetic analysis of bone marrow cells descendants of rats exposed to 131I males showed that chromosomal damage detected not statistically different from controls. Conclusions. Cytogenetic analysis showed the presence of chromosomal aberrations in the bone marrow cells of the first generation progeny. The frequency of chromosome aberrations was significantly higher due to specific markers of radiation exposure: paired fragments, dicentric and acentric rings. Results of the study confirmed the findings of other authors on the high sensitivity of the organism in the prenatal period to the mutagenic effects of radiation on the induction of chromosomal instability transgenerational.
Keywords: rats, iodine-131I, offsprings, the first generation, cytogenetic effects, bone marrow cells.