Expression pattern of SLWRKY33 and SLERF5 in tomato plants under elevated salt concentration and water deficit

  • H. R. Jafarov Khazar University,Azerbaijan, AZ-1009, Baku, Mahsati str., 11
  • K. G. Gasimov Institute of Biophysics, Azerbaijan National Academy of Sciences, Azerbaijan, AZ-1141, Baku, Z. Khalilov str., 117


Aim. High salinity, drought, and low temperature are the major abiotic stresses affecting plant growth and development and can lead to serious yield losses of agricultural crops. In plants, the majority of responses to abiotic stresses are con-trolled at the transcriptional level that is regulated by transcription factors (TFs). The family of WRKY and AP2/ERF are important superfamilies involved in response of plants to abiotic stress. Methods. The tissue-specific expression pattern of two superfamily members – SlWRKY33 from WRKY and SlERF5 from AP2/ERF were studied in tomato plants by means of Northern RNA hybridization and immunoblotting of total proteins. Results. Northern hybridization revealed very quick and strong expression of SlWRKY33 at elevated concentration of salt and water deficit, and rela-tively late and weaker expression of SlERF5 genes. Western hybridization with specific polyclonal antibodies revealed that during water deficit SlERF5 and SlWRKY33 were intensively expressed in variety of tissues of tomato plants. SlERF5 expressed in shoot apex, young leaves, roots and stems, while SlWRKY33 expressed in the same tissues as above and in axillary buds. Conclusions. Obtained results indicate that both SlWRKY33 and SlERF5 are involved in plant responses to abiotic stresses like elevated salt concentration and water deficit.
Keywords: Solanum lycopersicum L., transcription factor, cloning, immunohybridization abiotic stresses.


Yang B., Jiang Y., Rahman M.H., Deyholos M.K., Kav N.N.V. Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments. BMC Plant Biology.2009. 9:68. doi: 10.1186/1471-2229-9-68.

Li H., Gao Y., Xu H., Dai Y., Deng D., Chen J. ZmWRKY33, a WRKY maize transcription factor conferring enhanced salt stress tolerances in Arabidopsis. Plant Growth Regul. 2013. V.70. Р. 207–216.

Grennan A.K. Ethylene Response Factors in Jasmonate Signaling and Defense Response. Plant Physiology. 2008. V.146. P.1457–1458.

Lorenzo O., Piqueras R., Sanchez-Serrano J.J., Solano R., ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell. 2003. V.15. P. 165–178.

Jofuku K.D., Den Boer B.G., Van Montagu M., Okamuro J.K. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell. 1994. V.6(9). P. 1211–1225.

Ohme-Takagi M., Shinshi H. Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. Plant Cell. 1995. V.7(2). P. 173–182.

Okamuro J.K., Caster B., Villarroel R., Van Montagu M., Jofuku K.D. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. Natl. Acad. Sci. USA. 1997. V.94. P. 7076–7081.

Stockinger E.J., Gilmour S.J., Thomashow M.F. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE. Proc. Natl. Acad. Sci. USA. 1997. V.94. P. 1035–1040.

Gilmour S.J., Zarka D.G., Stockinger E.J., Salazar M.P., Houghton J.M., Thomashow M.F. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J. 1998. V.16(4). P. 433–442.

Kizis D., Pages M. Maize DRE-binding proteins DBF1 and DBF2 are involved in rab17 regulation through the drought-responsive element in an ABA-dependent pathway. Plant J. 2002. V.30(6). P. 679–689.

Vernie T., Moreau S., de-Billy F., Plet J., Combier J-P., Rogers C., Oldroyd G., Frugier F., Niebel A., Gamasa P. EFD Is an ERF Transcription Factor Involved in the Control of Nodule Number and Differentiation in Medicago truncatula. The Plant Cell. 2008. V.20. P. 2696–2713.

Baker S.S., Wilhelm K.S., Thomashow M.F. The 5'-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Mol Biol. 1994. V.24(5). P. 701–713.

Yamaguchi-Shinozaki K., Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell. 1994. V.6(2). P. 251–264.

Eulgem T., Rushton P.J., Robatzek S., Somssich I.E. The WRKY superfamily of plant transcription factors. Trends Plant Sci. 2000. V.5. P. 199–206.

Zheng Z., Qamar S.A., Chen Z., Mengiste T. Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant J. 2006. V.48. P. 592–605.

Journot-Catalino N., Somssich I.E., Roby D., Kroj T. The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana. Plant Cell. 2006. V.18. P. 3289–3302.

Kim K.C., Fan B., Chen Z. Pathogen-induced Arabidopsis WRKY7 is a transcriptional repressor and enhances plant susceptibility to Pseudomonas syringae. Plant Physiol. 2006. V.142. P. 1180–1192.

Knoth C., Ringler J., Dangl J.L., Eulgem T. Arabidopsis WRKY70 is required for full RPP4-mediated disease resistance and basal defense against Hyaloperonospora parasitica. Mol Plant Microbe Interact. 2007. V.20. P. 120–128.

Zou X. et al. A WRKY gene from creosote bush encodes an activator of the abscisic acid signaling pathway. J. Biol. Chem. 2004. V.279. P. 55770–55779.

Jiang Y., Deyholos M. Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses. Plant Molecular Biology. 2009. V.69(1). P. 91–105.

Gasimov K.G., Najafova L.A. SlWRKY33 promotes tomato plant tolerance to salt and drought stresses. Ukrainian Journal of Plant physiology and Genetic. 2014. V.46(5). P. 385–394.

Martínez-García J.F., Monte E., Quail P.H. A simple, rapid and quantitative method for preparing Arabidopsis protein extracts for immunoblotting analysis. Plant J. 1999. V.20(2). P. 251–257.

Yokota A., Takahara K., Akashi K. Water stress. Physiology and molecular biology of stress tolerance in plants. Edited by K.V. Madhava rao, A.S. Raghavendra, K. Janardhan Reddy. Springer. 2006. P. 15–40

Tan B.C., Joseph L.M., Deng W.T., Liu L., Li Q.B., Cline K., MaCarty D.R. Molecular characterization of the Arabidopsis 9-cis expoxycarotenoid dioxygenase gene family. Plant J. 2003. V.35. P. 44–56.

Assmann S.M., Snyder J.A., Lee Y.-R.J. ABA-deficient (aba1) and ABA-insensitive (abi1-1, abi2-1) mutants of Arabidopsis have a wild-type stomatal reponse to humidity. Plant Cell Environ. 2000. V.23. P. 387–395.