Получение трансгенных линий сахарной свеклы, содержащих синтетический ген cry1C

В. В. Курило, Е. Н. Шиша, А. И. Емец

Анотація


Aim. Insect pest’s impact makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into the plant genome is one of the promising strategies to ensure of plant resistance. The aim of this work was the production of sugar beet lines (based on the MM1/2 line) expressing cry1C genes. Methods. Genetic transformation of sugar beet was performed using the method of co-cultivation of leaf explants with Agrobacterium tumefaciens. Results. Sugar beet line MM1/2 was transformed by Agrobacterium-mediated method of transformation using binary vector pRD400-cry1CST, containing synthetic cry1C gene and selectable marker gene neomycin phosphotransferase II (nptII) conferring resistance to kanamycin. After the optimization protocol of genetic transformation and direct regeneration from leaf discs a transgenic sugarbeet lines were obtained. Conclusions. PCR analysis revealed integration of cry1C into the genome of transgenic lines of B. vulgaris.
Keywords: genetic transformation, Agrobacterium tumefaciens, Beta vulgaris, cry-genes.


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