The free proline levels in transgenic sunflower (Helianthus annuus L.) T3 plants with double-stranded proline dehydrogenase gene RNA-suppressor

  • A. G. Komisarenko
  • S. I. Mykhalska
DOI: https://doi.org/10.7124/FEEO.v20.766

Abstract

Aim. The investigation of the T3 transgenic sunflower plants osmotic tolerance there were developed. The levels of free proline in plant shoots and roots were estimated. Methods. Mature sunflower plants (T3 and wild type) were cultured in standard pots. Those genotypes were tested during 12-day artificial drying. The levels of free proline in plant shoots and roots were measured. Results. The proline contents in transgenic plants preferred those parameters of control plants both under normal and stress conditions. The proline levels in shoots and roots increased in all genotypes cultivated under stress conditions. The shoot/root proline ratio of control plants was constant during whole experiment, while in T3 plants this parameter changed due to high elevation in roots. Conclusions. The changes of shoot/root proline ratio of T3 plants were the result of free proline transfer among plant organs.

Keywords: Helianthus annuus L., transgenic plants, L-proline, shoot/root proline ratio.

References

Szabados L., Savoure A. Proline: a multifunctional amino acid. Trends in plant science. 2009. V.15(2). P. 89–97. doi: 10.1016/j.tplants.2009.11.009

Kavi Kishor P.B., Sangam S., Amrutha R.N., Sri Laxmi P., Naidu Rao K.R., Rao K.R.S.S., Rao S., Reddy K.J., Theriappan P., Sreenivasulu N. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implication in plant growth and abiotic stress tolerance. Current Sci. 2005. V.88(3). P. 424–438.

Miller G., Stein H., Honig A., Kapulnik Y., Zilberstein A. Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation. Planta. 2005. V.222. P. 70–79. doi: 10.1007/s00425-005-1518-4

Wang W., Vinocur B., Altman A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 2003. V.218. P. 1–14. doi: 10.1007/s00425-003-1105-5

Tishchenko E.N., Komisarenko A.G., Mikhal'skaia S.I., Sergeeva L.E., Adamenko N.I., Morgun B.V., Kochetov A.V. Agrobacterium-oposredovannaia transformatsiia podsolnechnika (Helianthus annuus L.) in vitro i in planta s ispol'zovaniem shtamma LBA4404, nesushchego plazmidu pBi2E s dvukhtsepochechnym RNK-supressorom gena prolindegidrogenazy. Tsitologiia i genetika. 2014. V.48(4). P. 19–30. [in Russian] doi: 10.3103/S0095452714040094

Serhieieva L.Ie., Mykhal's'ka S.I., Kurchiy V.M., Tyshchenko O.M. Vmist vil'noho prolinu v prorostkakh kukurudzy iak pokaznyk shvydkykh reaktsiy na diiu letal'nykh osmotychnykh stresiv in vitro. Fyzyologiia rasteniy i genetika. 2015. V.47(6). P. 491–496. [in Ukrainian]

Komisarenko A.H., Mykhal's'ka S.I., Tyshchenko O.M. Sposib zastosuvannia Agrobacterium-oposeredkovanoi transformatsii in planta dlia otrymannia transhennykh roslyn soniashnyka (Helianthus annuus L.). Patent Ukrainy na korysnu model' No 86108 vid 10.12.2013. Biul. No 23. [in Ukrainian]

Andriushchenko V.K., Saianova V.V., Zhuchenko A.A. et al. Modifikatsiia metoda opredeleniia prolina dlia vyiavleniia zasukhoustoychivykh form Lycopersicon Tourn. Izv. AN MSSR. 1981. V.4. P. 55–60. [in Russian]

Dospekhov B.A. Metody polevoy statistiki. M.: Agrokhimiia, 1985. 351 p. [in Russian]

Komisarenko A.H., Mykhal's'ka S.I., Kurchiy V.M., Tyshchenko O.M. Kharakterystyka transhennykh roslyn soniashnyka (Helianthus annuus L.) z dvolantsiuhovym RNK supresorom hena prolindehidrohenazy. Faktori eksperimental'noi evolucii organizmiv. 2016. V. 19. P. 143–147. [in Ukrainian]

Komisarenko A.G., Mikhal'skaia S.I., Kurchiy V.M., Sytnik S.K., Sergeeva L.E., Tishchenko E.N. Fiziologo-biokhimicheskaia kharakteristika transgennykh rasteniy podsolnechnika s dvukhtsepochechnym RNK-supressorom gena prolindegidrogenazy. Fiziologiia i biokhimiia rasteniy. 2015. V. 47(2). P. 160–166. [in Russian]