Біотехнологічні методи оцінки ефективності кріоконсервації сперми кнурів

С. І. Ковтун, О. І. Метлицька, О. В. Щербак, В. М. Гиря, К. В. Копилова

Анотація


Aim. Estimate boar semen for suitability to cryopreservation using informative biotechnological methods (thermoresistant probe, heat resistance test) to replenish genetic resources of Animal Gene bank Institute of Animal Breeding and Genetics n. M.V. Zubets, NAAS. Methods. In carrying out this work used biotechnology, cryobiological, morphological methods. Results. Mirgorod boars breed investigation we noted feature individual semen quality, which affect its suitability for cryopreservation. All boar genealogy analysis of experiment in Mirgorod breed descended from a line of boar named Lovchyk 1205, which was characterized by negative performance stability to semen cryopreservation. For morphometric and functional parameters of sperm, potentially of better quality sperm for cryopreservation was boar Mirgorod purebred № 1159, for which thermoresistant probe, heat resistance test indices was respectively 40.0 % and 35.0 %. Most suitable for cryopreservation in terms of heat resistance test boar semen was number 1159 and № 79, and amounted to 35.0 % and 30 %. Nevertheless, after thawing average sperm activity index fell to 83.7 %. Conclusions. Established a comprehensive method for assessing the suitability of native breeds boar semen cryopreservation involving of morphological and biotechnological methods

Keywords: cryobank, spermatozoa, cryopreservation, gene pool.


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