Аналіз осмостійкості трансгенних рослин пшениці, що містять ген орнітинамінотрансферази
Aim. Analysis of resistance to osmotic stress of transgenic wheat plant with a target gene ornithine aminotransferase. Methods. Physiological and biochemical methods were used to characterize the transgenic plants. Results. It is shown that increased expression of the gene oat accelerates rooting and encourages root growth is not only normal, but under stress. It is found that changes in gene expression oat not significantly affect the accumulation of proline neither normal nor under stress. Conclusions. It is shown that the introduction of genetic construct that increases expression of gene oat in wheat genome leads to increased osmotolerance of plants. Enhancement of gene expression oat not significantly affect the accumulation of proline neither normal nor under stress.
Keywords: Triticum aestivum, Agrobacterium-mediated transformation in vitro, ornithine aminotransferase, osmotolerance.