Визначення осмотолерантності рослин м’якої пшениці (Triticum aestivum L.), що містять дволанцюговий РНК-супресор гена проліндегідрогенази
Aim. Analysis of tolerance to osmotic stress of transgenic wheat plants, carrying a dsRNA-suppressor of prolinedegidrigenase gene. Methods. Physiological and biochemical methods were used to characterize the transgenic plants. Results. It is shown that partial suppression of gene prolinedegidrigenase was accompanied not only increase the level of free proline, but higher levels of resistance of transgenic wheat plants to water deficit. It is found that transgenic plants do not differ from control by morphological parameters and timing of development. Conclusions. The interrelation between catabolism of proline and resistance to osmotic stress was identified, which may be due to the influence of proline or the expression of other genes of plant stress response, or positive impact of increased resistance to proline content in the early stages of stress.
Keywords: Triticum aestivum, Agrobacterium-mediated transformation in vitro, dsRNA-suppressor proline dehydro-genase gene, osmotolerance.