Effect of low molecular weigt osmogenic substanses on the induction of embryogenesis in spring barley anther culture in vitro

  • O. V. Bilynska Plant Production Institute nd. a. V. Ya. Yuriev National Academy of Agrarian Sciences of Ukraine, Ukraine, 61060, Kharkiv, Moskovsky av., 142


Aim. Osmotic pressure of nutrient medium is known to be an important factor for morphogenesis in plant cell, tissue and organ culture in vitro. The investigations was aimed to elucidate the effect of maltose concentration and to evaluate the impact of mannitol add on callus, embryoid formation and plant regeneration in spring barley anther culture in vitro. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media contained N6 macro-, MS micronutrients, organic supplements, maltose (0−9.0 %) and 0.1 M mannitol. Results. A decrease in maltose concentration from 9 to 6 % had a strong negative effect on these processes. At the same time, addition of 0.1 M mannitol to medium containing 6 % maltose promoted sufficiently increase the efficiency of embryoid formation and plant regeneration. Conclusions. Medium osmotic pressure mainly determined by a high maltose concentration played an important role in spring barley haploid production. Decrease in maltose content could be compensated by addition of mannitol, which is osmotic substance with low metabolic activity.

Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, embryo formation, plant regeneration.


Sterward F.C., Mohn Ram H.Y. Determining factors in cell growth: some implications for morphogenesis in plant. Advan. Morphogenesis. 1961. V. 1. P. 180−266. doi: 10.1016/B978-1-4831-9948-1.50009-7

Vorob'ev L.N. Regulirovanie ionnogo transporta: teoreticheskie i prakticheskie aspekty mineral'nogo pitaniia rasteniy. Itogi nauki i tekhniki VINITI. Seriia Fiziologiia rasteniy. 1988. V. 5. 160 p. [in Russian]

Klarkson D. Trasport ionov i struktura rastitel'noy kletki / Per. s angl. M.G. Dudinoy; pod red. D.B. Vakhmistrova. Moskva: Mir, 1973. 368 p. [in Russian]

Lersten N.R. Flowering plant embryology. Ames: Blackwell Publishing, 2004. 212 p. doi: 10.1002/9780470752685

Biotekhnologiia rasteniy: kultura kletok / Per. s angl. V.I. Negruka; s predisl. R.G. Butenko. Moskva: Agropromizdat, 1989. 280 p. [in Russian]

Dunwell J.M. Anther and ovary culture. Cereal tissue and cell culture. Dordrech: Martinus Nijhof, 1985. P. 1–44. doi: 10.1007/978-94-009-5133-4_1

Finnie S.J., Powell W., Dyer A.F. The effect of carbohydrate composition and concentration on anther culture response in barley (Hordeum vulgare L.). Plant breeding. 1989. V. 103(2). P. 110–118. doi: 10.1111/j.1439-0523.1989.tb00358.x.

Sorvari S., Schider O. Influence of sucrose and melibiose on barley anther culture in starch media. Plant Breeding. 1987. V. 99(2). P. 161–171. doi: 10.1111/j.1439-0523.1987.tb01167.x

Kretovich V.L. Biokhimiia rasteniy: Ucheb. 2-e izd., pererab. i dop.; dlia biol. spets. un-tov. Moskva: Vyssh. shk., 1986. 503 p. [in Russian]

Abraimova O.Ie., Derkach K.V., Satarova T.M., Shevchenko N.V., Chernousova N.M. Vplyv manitolu na induktsiiu ta reheneratsiiu morfohennoi kalusnoi tkanyny u kukurudzy. Faktory eksperymental'noi evoliutsii orhanizmiv. 2016. V. 17. P. 103–106. [in Ukrainian]

Kasha K.J., Simion E., Oro R., Yao Q.A., Hu T.C., Carlson A.R. An improved in vitro technique for microspore culture of barley. Euphytica. 2001. V. 120(3). P. 379–385. doi:10.1023/A:1017564100823.

Roberts-Oehlschlager S.L., Dunwell J.M. Barley anther culture: pretreatment on mannitol stimulates production of microspore-derived embryos. Plant Cell, Tissue and Organ Culture. 1990. V. 20(3). P. 235–240. doi: 10.1007/BF00041887.

Shim Y.S., Kasha K.J. The influence of pretreatment on cell stage progression and the time of DNA synthesis in barley (Hordeum vulgare L.) uninucleate microspores. Plant Cell Reports. 2003. V. 21(11). P. 1065–1071. doi: 10.1007/s00299-003-0635-4.

Bergen S., Wang M. Microspore regeneration as a tool for plant breeding. Acta Horticulture. 2002. No. 572. P. 51–57. doi: 10.17660/ActaHortic.2002.572.5.

Belinskaia E.V. Vliianie predobrabotki kolos'ev na effektivnost' induktsii gaploidov iachmenia v kul'ture pyl'nikov in vitro. Fiziologiia i biokhimiia kul'turnykh rasteniy. 2005. V. 37(5). P. 436–442. [in Russian]

Bilyns'ka O.V. Pidvyshchennia efektyvnosti eksperymental'noho androhenezu in vitro u iachmeniu shliakhom optymizatsii poperedn'oi obrobky kolossia v umovakh nyz'koi pozytyvnoi temperatury. Faktory eksperymental'noi evoliutsii orhanizmiv: zb. nauk. prats. K.: Lohos, 2006. V. 3. P. 437–444. [in Ukrainian]

Belinskaia E.V. Vliianie elementov tekhnologii gaploidnoy induktsii na proiavlenie genotipicheskikh osobennostey morfogeneza v kul'ture pyl'nikov in vitro iarovogo iachmenia. Tsitologiia i genetika. 2010. V. 44(2). P. 38–44. [in Russian]

Belinskaia E.V., Dul'nev P.G. Modifitsirovannyy krakhmal kak komponent pitatel'noy sredy dlia polucheniia gaploidov iachmenia v kul'ture pyl'nikov in vitro. Fiziologiia i biokhimiia kul'turnykh rasteniy. 2007. V. 39(2). P. 136–143. [in Russian]

Murashige T., Skoog F. A revised medium for growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962. V. 15. P. 473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x.