Meiotic mutants tomatoes with desynaptic phenotype

  • T. P. Lisovska Lesia Ukrainka Estern European National University, Ukraine, 43025, Lutsk, Voli prosp., 13


Aim. A comparative analysis of the cytological manifestation in the meiosis of six meiotic mutants was carried out. Methods. Buds with anthers 2–3 mm long fixed in ethanol: glacial acetic acid in a ratio of 3:1, were stored in 70 % ethanol, stained with acetocarmine. For cytology preparations prepared pressure anthers at various stages of meiosis. Pollen fertility was determined by staining with acetocarmine. Results. Meiotic mutations tomato dsm1, dsm2, dsm3, as1, as5 and asb not affect the vegetative growth of plants, but have low fertility of pollen and number of seeds in the fruit. They are similar cytological manifestation. The beginning of meiosis occurred apparently normal, as violations of premature decay bivalent observed since diplotenе. Chiasma frequencies in pollen mother cells ranged from 8.13 to dsm3 / dsm3 to 6.73 in asb / asb. All investigated mutations revealed monogenic recessive character of inheritance. Mutations as1, as5 and asb and dsm2 are not allelic to each other. The mutation dsm1 appeared allelic to mutations dsm2 and asb. Conclusions. Investigated meiotic mutations failure of chiasma maintenance in the pollen mother cells with varying degrees of desynapsis. Based on the fact that the previously published data did not reveal a reduction in the recombination frequency in marked areas of the genome, we anticipate premature removal of cohesin that hold sister chromatids distal chiasmata.
Keywords: meiosis, meiotic mutants, sister chromatids cohesion, premature separation of the bivalents, Lycopersicon esculentum Mill.


Zamariola L., Tiang C.L., De Storme N., Pawlowski W., Geelen D. Chromosome segregation in plant meiosis. Front. Plant Sci. 2014. V. 5, Article 279. doi: 10.3389/fpls.2014.00279.

Mercier R., Mezard Ch., Jenczewski E., Macaisne N., Grelon M. The molecular biology of meiosis in plants. Annu. Rev. Plant Biol. 2015. V. 66 P. 5.1–5.31. doi: 10.1146/annurev-arplant-050213-035923.

Peters J.-M., Tedeschi A., Schmitz J. The cohesin complex and its roles in chromosome biology. Genes & Development. 2008. V. 22. P. 3089–3114. doi: 10.1101/gad.1724308

De K., Sterle L., Krueger L., Yang X., Makaroff C.A. Arabidopsis thaliana WAPL is essential for the prophase removal of cohesin during meiosis. PLoS Genet. 2014. 10 (7). e1004497. doi: 10.1371/journal.pgen.1004497.

Lisovs'ka T.P., Voytiuk V.P., Kuz'mishyna I.I. Tsytolohichnyy i henetychnyy analiz meyotychnoi mutatsii tomatu dsm1. Naukovyy visnyk VNU im. Lesi Ukrainky. Biolohichni nauky. 2010. No 12. P. 105–111. [in Ukrainian]

Soost R.K. Comparative cytology and genetics of asynaptic mutants in Lycopersicon esculentum Mill.. Genetics. 1951. V. 36. P. 110–134.

Moens P.B. Genetic and cytological effects of three desynaptic genes in the tomato. Canad. J. Genet. and Cytol. 1969. V. 11(4). P. 857–859. doi: 10.1139/g69-101

Lisovs'ka T.P., Stratu L.S., Voytiuk V.P., Dmytrotsa O.R. Chotyry meyotychni mutatsii tomatu redukuiut' chastotu khiazm i vyiavliaiut' normal'nyy abo vysokyy riven' homolohichnoi rekombinatsii. Dosiahnennia i problemy henetyky, selektsii ta biotekhnolohii: Zb. nauk. prats'. / Ukr. T-vo henetykiv i selektsioneriv im. M.I. Vavylova. K.: Lohos, 2007. Vol. 1. P. 109–113. [in Ukrainian]

Havekes F.W., de Jong J.H., Heyting C., Ramanna M.S. Synapsis and chiasma formation in four synaptic mutants of the tomato (Lycopersicon esculentum). Chromosome Research. 1994. V. 2. P. 315–325. doi: 10.1007/BF01552725

Enyenihi A.H., Saunders W.S. Large-Scale Functional Genomic Analysis of Sporulation and Meiosis in Saccharomyces cerevisiae. Genetics. 2003. V. 163(1). P. 47–54.

Grishaeva T.M., Bogdanov Iu.F. Geneticheskiy kontrol' meyoza u drozofily. Genetika. 2000. Vol. 36(10). P. 1301–1321. [in Russian]

Mercier R., Grelon M. Meiosis in plants: ten years of gene discovery. Cytogenet. Genome Res. 2008. V. 120. P. 281–290. doi: 10.1159/000121077