Cellulolytic and xylanolytic enzyme complex of Penicillium funiculosum Thom

  • О. М. Юр’єва
  • І. М. Курченко
  • С. О. Сирчін
  • О. С. Харкевич
  • А. К. Павличенко
  • Л. Т. Наконечна

Abstract

Aim. The aim of this research was a comparative study of cellulase and xylanase activities of endophytic and soil Penicillium funiculosum strains. Methods. Endoglucanase activity was determined by hydrolysis of 2.0 % solution of Na-CMC, exoglucanase – filter paper and xylanase – beech xylan. Reducing substances were estimated using DNS method. Results. Obtained data demonstrate the ability of endophytic and soil P. funiculosum strains to synthesize complex of cellulolytic and xylanolytic enzymes and hydrolyze the synthetic substrate Na-CMC. Studied activities enhanced with increasing cultivation time of micromycetes. Endophytic isolates of P. funiculosum had lower exo-, endoglucanase and xylanase activities than soil ones. Conclusions. The fact that endophytic and saprophytic P. funiculosum strains synthesize hydrolytic enzymes allows screening them further for producers of hydrolytic enzyme complexes and development of modern biotechnology transformation of lignocellulosic substrates.

Keywords: Penicillium funiculosum, endophytes, saprophytes, cellulase and xylanase activities.