Аналіз просторової організації актинових мікрофіламентів у відповіді рослин на дію холоду за використання програми MicroFilament analyzer
Aim. The paper demonstrated the use of MicroFilament analyzer (MFA) program to study the organization of actin filaments in different cells of Arabidopsis roots under cold action. Methods. For visualization of actin filaments in different types of root cells of A. thaliana (GFP-ABD2-GFP) seedlings the confocal laser scanning microscope LSM 510 META was used. To analyze the microfilaments’ orientation after exposure to temperatures +4о and +0.5оC MFA software has been applied. Results. It has been found that low temperatures effect the organization and the orientation of native microfilaments in different cell types of the main Arabidopsis roots. The analysis by MFA showed that cold stress and exposure time are important factors affecting the orientation of actin filaments. Conclusions. Using MFA software to determine the effects of low temperatures on microfilaments is essential for a more accurate and detailed understanding of cold effect on the behavior of these filamentous structures in Arabidopsis in a quickly and standardized manner.
Keywords: actin filanents, root apex, low temperature, MicroFilament analyzers (MFA), A. thaliana лінії GFP-ABD2-GFP.