Investigation of dermal equivalents efficacy for the treatment of severe burn wounds

Abstract

Aim. The aim was to estimate the efficiency of a new dermal equivalent in vivo. Methods. We carried out the application of wound coverages containing hydrogels with mesenchymal stem cells (MSCs) on the third degree burns of ICR line mice. The duration of application was 8 hours. In the control group animals had no treatment. Photofixation of burn wound station was carried out once a day. The results were estimated by two-way ANOVA approach. Results. There was a statistically significant difference of burn wounds development on the third day after treatment in experimental groups of animals comparing with the control. Clinical investigations realized at the Center of thermal burns and plastic surgery confirmed beneficial effects of our dermal coverages on skin regeneration. In patients after treatment processes of vascularization and epithelialization of the wound started earlier and efficiency of autografts engraftment was improved. Conclusions. It has been shown high effectiveness of dermal equivalents containing MSCs in the experiments with animals in vivo and in clinicum.

Keywords: burn wound, dermal equivalent, skin equivalent, skin substitute, tissue engineering.