Характеристика трансгенних рослин соняшника (Helianthus annuus L.) з дволанцюговим РНК супрессором гена проліндегідрогенази
Aim. The obtaining of osmotolerant form of sunflower (Helianthus annuus L.) by siRNA-technology. Methods. Agrobacterium-mediated transformation using LBA4404 harboring pBi2E with double-stranded RNA-suppressor, produced based on the ProDH1 gene of arabidopsis. Gene exprssion of proline dehydrogenase at level of activity of this ferment. Results. The transgenic sunflower plants with dsRNA suppressor proline dehydrogenase gene and their seed generation were obtained via Agrobacterium-mediated transformation in planta. Thransgenic osmotolerant plants had lower activity (6 times less) of proline dehydrogenase as well as increased level of free proline.
Keywords: Helianthus annuus L., genetic transformation, dsRNA suppressor proline dehydrogenase gene, osmotic stress, proline, proline dehydrogenase, activity of proline dehydrogenase.