IRAP and REMAP techniques for polymorphism detection in amphidiploids which are instable for glaucousness

  • V. V. Shpylchyn National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2
  • T. K. Ternovska National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2

Abstract

Aims. To study polymorphism of amplification products derived from the DNA of amphidiploids which are contrast for glaucousness with primers of IRAP and REMAP systems. Methods. PCR with primers to LTR of retrotransposons and microsatellites. Electrophoresis of DNA fragments. Results. DNA amplification of investigated plants produced monomorphic or polymorphic components of spectrum depending on amphidiploid genome, AABBTT or AABBMtMt and primers used. LTR/LTR primers in this plant material were detected as more informative as LTR/SSR primers. Conclusions. In this plant material the polymorphism for amplification products with primers to LTR of retrotransposons for pair Nikita/Nikita, Sukkula/Sukkula, Sabrinai/Sabrina were detected.

Key words: Triticinae, retrotransposons, glaucousness, amphidiploids.