Genotyping Triticum aestivum L. cultivars of Ukraine with microsatellite markers

Abstract

Aim. To develop an evaluation system of genetic polymorphism for wheat cultivars of Ukrainian origin based on analysis of SSR loci. Methods. PCRs with the following separation of amplification products by electrophoresis in agarose (2 %) were performed to find out genetic polymorphism. Phylogenetic relationship was identified with UPGMA method. Results. The dendrogram of phylogenetic relationships for 15 wheat cultivars was constructed. Conclusions. It is turned out that two wheat cultivar Podolianka and Bohdana had very similar genotype within the analysis. It is necessary to carry out the further research to establish similarity or diversity between them.

Keywords: Triticum aestivum L., SSR markers, dendrogram, phylogenetic relationship.