Awn promoter genes in genomes of Triticinae

  • T. K. Ternovska National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2
  • M. Z. Antonyuk National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2
  • V. S. Martynenko National University of Kyiv-Mohyla Akademy, MONMS Ukraine Ukraine, 04070, Kyiv, G. Skovorody str., 2

Abstract

Aims. Identification of Triticinae group of homoeologous chromosomes in which awn promoter is localized in Aegilops species. Determination of genetic control of awns development in wheat-goatgrass introgressive lines. Methods. Visual assessment, study of chromosome configurations at meiosis M1 in PMCs, protein electrophoresis in PAGE, PCR with primers to chromosome-specific SSR loci, statistical methods. Results. Association between electrophoretic spectra components controlled by 6 group chromosomes and awn development gradations has been determined. Awn promoter awnP has been localized in the homoeologous chromosomes group 6 in goatgrass. Hybridological analysis has shown semi-epistatic nature of B1 gene as to awnP and bn genes. Awned lines have deletion or mutation of В1 gene. Conclusions. Gene-promoter awnP is localized in Ssh, S, U chromosomes of tree Aegilops species, and this gene hypostatic to dominant inhibitor of awn development B1. Gene bn in 6D chromosome is also hypostatic to В1 gene.

Key words: Awn development, SSR-loci, Triticinae, goatgrass.