Novel genes that govern production of phosphoglycolipid family antibiotics

  • Б. О. Осташ
  • Л. О. Горбаль
  • Н. В. Забуранний
  • М. М. Лопатнюк
  • Г. В. Мутенко
  • М. В. Рабик
  • О. М. Громико
  • І. С. Осташ
  • С. Уокер
  • В. О. Федоренко

Abstract

Aims. To identify novel genes for biosynthesis of moenomycin-like natural products in actinomycetes, and genes that control their production at transcriptional and posttranscriptional levels. Methods. Bionformatic genome mining was combined with experimental verification of selected genes; gene knockouts and erexpression studies were used for the latter. Results. A genetic network putatively controlling the production of moenomycins by Streptomyces ghanaensis ATCC14672 is described. Involvement of several genes from this network was verified experimentally. Gene cluster for biosynthesis of biosynthesis of putative phosphoglycolipid natural product was identified in the genome of Actinoplanes teichomyceticus, and this cluster features both novel genetic architecture and novel genes for phosphoglycolipid biosynthesis. Conclusions. The obtained data create a working ground for development of moenomycin overproducers and generation of producers of novel moenomycins.

Keywords: Streptomyces, antibiotics, phosphoglycolipid, moenomycin.