Hobo transposon in the natural populations of Drosophila melanogaster in Ukraine

  • D. I. Kozeretska ESC “Institute of Biology”, National Taras Shevchenko University of Kyiv Ukraine, 01601, Kyiv, Volodymyrska str, 64
  • S. V. Serga ESC “Institute of Biology”, National Taras Shevchenko University of Kyiv Ukraine, 01601, Kyiv, Volodymyrska str, 64
  • A. S. Demydova ESC “Institute of Biology”, National Taras Shevchenko University of Kyiv Ukraine, 01601, Kyiv, Volodymyrska str, 64
  • S. E. Shkliar ESC “Institute of Biology”, National Taras Shevchenko University of Kyiv Ukraine, 01601, Kyiv, Volodymyrska str, 64
  • I. A. Kozeretska ESC “Institute of Biology”, National Taras Shevchenko University of Kyiv Ukraine, 01601, Kyiv, Volodymyrska str, 64

Abstract

Aims. The goal of our study was to investigate population dynamics of the hobo transposon in the wild population of Drosophila melanogaster in Ukraine. Methods. Samples from the wild populations were collected across 11 different locations. Presence of the hobo element was determined using PCR with following sequencing. ClustalW and VectorNTI 11.0 software was used for sequence comparison. Results. We have found fragments of hobo transposon in all studied populations of D. melanogaster. Alignment test provides the evidence of identity of the studied element with the canonical sequence, that suggests the invasive pattern of this transposon in sampled populations' genomes.

Key words: Drosophila melanogaster, hobo transposon, natural populations.