Поліморфізм інтрогресивних ліній м’якої пшениці за алелями мікросателітних локусів
Aims. Evaluation of possibilities to use microsatellite loci as potential molecular markers of alien chromatin in the genomes of wheat introgressive lines, which contain genetic material from Ae. speltoites, Ae. sharonensis, Ae. umbellulata and are resistant to powdery mildew. Evaluation of acceptability of SSR analysis for specification of introgressive lines genome structure. Methods. PCR of genome DNA of wheat genotypes for identification of microsatellite loci specific to 3D chromosome. Results. The identified polymorphisms of SSR loci were represented by new polymorphic variants, which are different from parental genotypes used for F1 development and by heterozygotes. Microsatellite loci located more distal to centromere were shown to be more polymorphic. Conclusions. Polymorphism of SSR loci limits the potential of their use for investigation of introgressive lines genome structure. Microsatellites can be used for screening segregating populations taking into consideration the possibility of new allele appearance.Key words: wheat, introgressive lines, powdery mildew, microsatellites.