Introgressive wheat lines Triticum aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes

Abstract

Aims. Using SSR loci specific to homeological group 5 chromosomes, identify T genome introgressions in the genomes of F₅ wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identified 9 SSR loci specific to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F₅ progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a significant number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from F₂. Conclusions. Microsatellite loci could be used for identification of introgressions from Aegilops mutica in wheat genome (although with some limitations). Identified plants with recombinant 5D/5Т chromosomes are perspective for future work.

Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines, recombinant lines.