Introgressive wheat lines Triticum aestivum/Amblyopyrum muticum as to structure of homoeologous group 5 chromosomes
Aims. Using SSR loci specific to homeological group 5 chromosomes, identify T genome introgressions in the genomes of F5 wheat plants from crossing Aurora x Aurotica. Methods. DNA extraction using CTAB method, PCR with primers to SSR loci, electrophoresis in PAAG, silver staining. Results. Comparative microsatellite analysis of Aurora and Aurotica identified 9 SSR loci specific to D genome which produced differing amplicons with Aurotica DNA. These loci were used for screening (Aurora x Aurotica) F5 progeny (234 plants); 24 plants with disomic substitutions 5D/5T were detected, and a significant number of plants containing recombinant chromosome 5D/5T. The recombination event could occur in any generation from F2. Conclusions. Microsatellite loci could be used for identification of introgressions from Aegilops mutica in wheat genome (although with some limitations). Identified plants with recombinant 5D/5Т chromosomes are perspective for future work.
Keywords: introgressive hybridization, Amblyopyrum muticum, microsatellite analysis, SSR loci, alien-substitution lines, recombinant lines.