Хромосомная нестабильность в облучённых лимфоцитах периферической крови человека при совместном культивировании с клетками дрожжей Saccharomyces cerevisiae
Aims. In our previous studies we have shown 3-4 times increasing of the aberrant metaphases level in intact human lymphocytes as a result of bystander effect (BE) induced by irradiated yeast cells. The question remains are damaged cells more sensitive to BE signals or not? The purpose of our study was investigation how cocultivation with S. cerevisiae affects the level of chromosomal instability in X-ray irradiated lymphocytes. Methods. The cultures of X-ray irradiated (1 Gy) human peripheral blood lymphocytes were experimentally contaminated with nonirradiated or X-ray irradiated yeast cells (haploid strains of S. cerevisiae, 10 Gy). Well spread human metaphases were scored for aberration metaphases (AM). Results. It was found that irradiated yeast cells had no effect on chromosomal stability level in irradiated lymphocytes. The statistically significant decreasing of the AM level was observed in irradiated lymphocytes incubated with non-irradiated yeast cells. Conclusions. Our findings suggest existence of “inverse BE” when non-irradiated cells can significantly reduce the level of chromosomal damages in irradiated ones.Key words: bystander effect, lymphocytes, aberration metaphases.