Features of exosom isolation from tin lacquered mycelium

  • M. Palladin Institute of Food Biotechnology and Genomics, Natl. Acad. Sci. Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2А
  • A. I. Yemets Institute of Food Biotechnology and Genomics, Natl. Acad. Sci. Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2А https://orcid.org/0000-0001-6887-0705
DOI: https://doi.org/10.7124/FEEO.v37.1768
Keywords: Ganoderma lucidum (Curtis) P. Karst., mycelium, exosomes, nanovesicles

Abstract

Aim. To apply the proposed methods for obtaining exosomes and select conditions for effective isolation of these nanovesicles from the mycelium of the fungus Ganoderma lucidum (Curtis) P. Karst. To obtain exosomes from the cells of the mycelium of G. lucidum and to conduct their morphometric analysis. Methods. Microbiological, microscopic, sedimentation, and spectrophotometric research methods were used to obtain and analyze isolated exosomes from the mycelium of the G. lucidum fungus.. Results. Various methods for isolating exosomes were tested and an effective method for grinding, homogenizing, and purifying exosomes from G. lucidum mycelium cultivated in vitro was selected. The isolation and primary identification of exosomes from G. lucidum mycelium was performed for the first time. Conclusions. The developed approach for isolating exosomes from the mycelium of the fungus G. lucidum grown under stationary aseptic conditions in vitro is an important step for further investigation the biological activity of these nanovesicles and the possibility of their practical application.

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