Pro-apoptotic activity of the antiviral and antitumor drug Izatizon
Abstract
Aim. Apoptosis is a programmed cell death characteristic of all multicellular organisms, which is not accompanied by an inflammatory process, unlike necrosis, and is an immunologically inert process. To study the proapoptotic effect of isathizone and its analogues on the culture of the human malignant cell line K-562. Methods. Flow cytometry was used to detect the effects of drugs on apoptosis. The effect of drugs on caspase-3 activity was determined using Western blot. Results. Flow cytometry data show that isathizone and its analog Idd stopped cell division in the G0–G1 phase, after which apoptosis began. The data of studies using flow cytometry show that isatizone and its analogue Idd stopped cell division in the G0-G1 phase, after which apoptosis began. Studies on the effect of isathizone and its analogues on the activity of caspase-3 in the culture of malignant K-562 cells showed that all drugs initiate caspase-3. Conclusions. Izatizone and its analogues affect apoptosis of malignant K-562 cell cultures and can be used for further research in practical medicine.
References
Zaika L. A., Bolsunova O. I., Potopalskyi A. I. Protyvirusni, protypukhlynni ta imunomoduliuiuchi vlastyvosti likuvalnoho preparatu Izatizon. Kyiv : Kolohib, 2010. P. 27–36.
Maheshwari A., Thuluvath R. Acute hepatitis C. Lancet. 2008. Vol. 372. P. 321–342.
Bolsunova O. I., Brovarets O. O., Hovorun D. M., Zaika L. A., Potopalsky A. I. Quantum Chemical Analysis of Structural and conformational Properties of Methisazone and Prototropic Tautomerism of Isatin. IREBIC. 2011. Vol. 2(5). P. 159–165..
Potopalskyi A. I., Loziuk L. V. Protyvovirusnyi, protyvopukholevyi preparat Izatizon. Kyiv : Naukova dumka, 1995. P. 57–64.
Patskovsky U. V., Negrebetskaya E. N., Chernomas A. A., Voloschuk T. P., Kitam O. E, Rubashevsky E. I., Tereschenko M. J., Nosach I. N., Potopalsky A. I. Aromatic thiosemicarbasones: their antiviral action and interferon / The decreasing of adenovirus type-1 resistance against interferon by methisazone in vitro. Biopolymers & Cell. 1996. Vol. 12(2). Р. 74–83.
Van Parijs, Abbaa A. K. Homeostasis and self-tolerance in the immune system: turning lymphocytes off. Science. 1998. Vol. 280. P. 243–248.
Bolsunova O. I., Rybalko S. L., Zaika L. A., Potopalskyi A. I. Vyvchennia mekhanizmiv dii preparatu Izatizon na reparatyvnu spromozhnist klityn pry eksperymentalnii herpetychnii infektsii. XIX Mizhnarodna internet-konferentsiia «Veterynarni, silʹsʹkohospodarsʹki, biolohichni, khimichni nauky: stan ta perspektyvy rozvytku v XXI stolitti» (15–20 liutoho 2012).
Khomenko A. V. Hidroksyliuvannia kholekaltsyferolu v hepatsytakh shchuriv pid diieiu prednizolonu. Ukrainskyi biokhimichnyi zhurnal. 2013. Vol. 85(3). P. 73–78.
