Characterisation of winter bread wheat lines with the 1BL.1RS translocation of the vyshyvanka type

  • N. O. Kozub Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0000-0002-3572-1786
  • I. O. Sozinov Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33 https://orcid.org/0000-0002-3621-5746
  • N. O. Demianova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0009-0009-4712-8275
  • O. I. Sozinova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0000-0002-0981-3433
  • Y. B. Blume Institute of Food Biotechnology and Genomics, Natl. Acad. Sci. Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2А https://orcid.org/0000-0001-7078-7548
DOI: https://doi.org/10.7124/FEEO.v37.1741
Keywords: Triticum aestivum L., secalins, powdery mildew resistance, spike traits, rolled leaf

Abstract

Aim. We identified a new 1BL.1RS translocation in the spring bread wheat variety Vyshyvanka using secalin loci. The aim of the work was to develop winter lines with this translocation and to study their characteristics. Methods. The winter varieties Samurai and Solomiya were crossed with Vyshyvanka. To select homozygotes for the translocation, acid electrophoresis of gliadins and SDS-electrophoresis were performed. For the family of F5 lines from the cross Samurai x Vyshyvanka, yield-related traits of the main spike were determined. Results. The analysis of segregation in F2 seeds from the cross Vyshyvanka x Solomiya showed a reduced frequency of transmission of the translocation through pollen grains. Via marker-assisted selection, we developed winter lines with the Vyshyvanka-type translocation that were resistant to powdery mildew against the natural background. The F3–F5 lines derived from F2 spike 175-1 from the cross Samurai x Vyshyvanka showed a high degree of leaf rolling Those F5 lines had a higher number of spikelets and grains per spike than Samurai but did not differ in grain weight per spike. Conclusions. Winter lines from crosses with Vyshyvanka are promising breeding material with the new 1BL.1RS translocation with resistance to powdery mildew, high spike yield-related traits and, in the case of mutants, the rolled leaf character for the development of drought tolerant varieties.

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