The induction of non-specific immunity of wheat by UV-C treatment and kojic acid as a biotic elicitor

Keywords: Triticum aestivum, Mucor sp., UV-C irradiation, kojic acid, hydrogen peroxide

Abstract

Aim. Plant protection needs a holistic approach and climate changes escalate the problems of important agricultures growing. The aim of our research was to induce the non-specific tolerance of wheat Triticum aestivum to pathogens by UV-C irradiation and kojic acid as a biotic elicitor. Methods. Seeds and plants of winter wheat cv Podolyanka were irradiated by Philips TUV 30 W lamp. The contamination was evaluated and phytopathogen identified. The plants of wheat cv Podolyanka were sprayed by 0.1 mM kojic acid water solution at the second leaf stage. During the experiments the ehdogenous hydrogen peroxide content and morphometric parameters of leaves and roots were measured. Results. It is demonstrated that the effectiveness of UV-C irradiation was more significant with lower doses, the optimal is between small doses with the stimulation influence and higher ones. It is shown that the hydrogen peroxide content in infected winter wheat leaves Triticum aestivum cv Podolyanka after this combination was high during the first days of experimental period and later stabilized. Conclusions. It is detected that combination of UV-C irradiation with kojic acid treatment maintained the plant growth and the development, accelerate their repair and decreased the damage made by phytopathogen Mucor sp.

References

Janni M., Maestri E., Gullì M., Marmiroli M., Marmiroli N. Plant responses to climate change, how global warming may impact on food security: a critical review. Front. Plant Sci. 2024. Vol. 14. 1297569. doi: 10.3389/fpls.2023.1297569.

Förderer A., Li E., Lawson A. W. et al. A wheat resistosome defines common principles of immune receptor channels. Nature. 2022. Vol. 610. P. 532–539 doi: 10.1038/s41586-022-05231-w.

Dodds P. N., Chen J., Outram M. A. Pathogen perception and signaling in plant immunity. Plant Cell. 2024. doi: 10.1093/plcell/koae020.

Ding L. N., Li Y. T., Wu Y. Z., Li T., Geng R., Cao J., Zhang W., Tan X. L. Plant disease resistance-related signaling pathways: recent progress and future prospects. Int. J. Mol Sci. 2022. Vol. 23 (24). 16200. doi: 10.3390/ijms232416200.

Zhuk І. V., Dmitriev A. P., Lisova G. M., Kucherova L. The influence of kojic acid and donor NO on Triticum aestivum L. under biotic stress. Factors in experimental evolution of organisms. 2019. Vol. 24. P. 219–224. doi: 10.7124/FEEO.v25.1166. [in Ukranian]

Zhuk І. V., Dmitriev A. P., Shylina Ju.V., Lisova G. M., Kucherova L.O. The estimation of organic acids effectiveness as biotic elicitors via changes of endogenous peroxide content. Factors in experimental evolution of organisms. 2020. Vol. 26. P. 202–206. doi: 10.7124/FEEO.v26.1266. [in Ukranian]

Zhuk І. V., Shylina Ju. V., Dmitriev A. P. Effect of biotic elicitor and donor NO treatment in complex defence of wheat plants against hypoxia and wounding stress. Factors in experimental evolution of organisms. 2022. Vol. 30. P. 73–78. doi: 10.7124/FEEO.v30.1464. [in Ukranian]

Sokolova D. A., Halych T. V., Zhuk V. V., Kravets A. P. Involvement of UV-C-induced genomic instability in stimulation рlant long-term protective reactions. J. Plant. Physiol. 2024. Vol. 293. 154171. doi: 10.1016/j.jplph.2024.154171.

Vanhaelewyn L., Van Der Straeten D., De Coninck B., Vandenbussche F. Ultraviolet radiation from a plant perspective: the plant-microorganism context. Front Plant Sci. 2020. Vol 15, 11. 597642. doi: 10.3389/fpls.2020.597642.

De Caldas Felipe M. T., do Nascimento Barbosa R., Pereira Bezerra J. D., de Souza-Motta C. M. Production of kojic acid by Aspergillus species: Trends and applications, Fungal Biology Reviews. 2023. Vol. 45. doi: 10.1016/j.fbr.2023.100313.

Macedo W. R., Silva G. H., Santos M. F. C., Oliveira A. P. S., Souza D. S. Physiologic and metabolic effects of exogenous kojic acid and tyrosol, chemicals produced by endophytic fungus, on wheat seeds germination. Natural Product Research. 2018. Vol. 32 (22). P. 2692–2696. doi: 10.1080/14786419.2017.1374261.

Hassan M., Shahzadi S., Kloczkowski A. Tyrosinase inhibitors naturally present in plants and synthetic modifications of these natural products as anti-melanogenic agents: a Review. Molecules. 2023. Vol. 28. P. 378. doi: 10.3390/molecules28010378.

Chen L.-M., Kao Ch.-H. Effect of excess copper on rice leaves: evidence involvement of lipid peroxidation. Bot. Bull. Acad. Sin. 1999. Vol. 40. P. 283–287.

Zhu A., Liu M., Tian Z. et al. Chemical-tag-based semi-annotated metabolomics facilitates gene identification and specialized metabolic pathway elucidation in wheat. Plant Cell. 2024. Vol. 26, 36 (3). P. 540–558. doi: 10.1093/plcell/koad286.

Dixon R. A., Dickinson A. J. A century of studying plant secondary metabolism – From “what?” to “where, how, and why? Plant Physiol. 2024. Vol. 195 (1). P. 48–66. doi: 10.1093/plphys/kiad596.