Characterization of Bila Tserkva winter common wheat cultivars with respect to marker loci

  • N. O. Kozub Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0000-0002-3572-1786
  • I. O. Sozinov Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33 https://orcid.org/0000-0002-3621-5746
  • H. Ya. Bidnyk Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0009-0002-9521-7618
  • N. O. Demianova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0009-0009-4712-8275
  • O. I. Sozinova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0000-0002-0981-3433
  • I. I. Kucheriavyi Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33
  • O. I. Borzykh Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33 https://orcid.org/0000-0002-9802-5622
  • Y. B. Blume Institute of Food Biotechnology and Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy Vyshnevetskoho str., 2A https://orcid.org/0000-0001-7078-7548
DOI: https://doi.org/10.7124/FEEO.v35.1666
Keywords: Triticum aestivum, storage proteins, disease resistance genes, 1BL.1RS

Abstract

Aim. We studied special features of the sample of Bila Tserkva winter common wheat cultivars with respect to storage protein loci and some disease resistance genes. Methods. To identify alleles at gliadin and high-molecular-weight glutenin loci, acid polyacrylamide gel electrophoresis and SDS-electrophoresis of grain proteins were carried out. Alleles of markers for the disease resistance genes Lr34, Tsn1, and TDF_076_2D were identified using PCR. Results. The composition and frequencies of alleles at the Gli-1, Glu-1, and Gli-A3 loci were studied in samples of Bila Tserkva cultivars released before 2011 and after 2010. For the latter group of cultivars, alleles of the disease resistance gene markers were identified. We observed a significant increase in the frequency of the allele Gli-A1x(9) and a decrease in the frequency of Gli-A1c, as well as a tendency for an increase in the frequency of Gli-A3c. In the total sample of Bila Tserkva cultivars, nonrandom associations of pairs of certain storage protein alleles were revealed. Conclusions. The group of Bila Tserkva cultivars is similar to the previously studied groups of the Central Forest Steppe of Ukraine with respect to allele set and frequencies of the main prolamin loci, but differs in the frequencies of the disease resistance genes. A special feature of the group of Bila Tserkva cultivars is a high frequency of the allele c at the minor locus Gli-A3, as well as the allele association Gli-A1x Gli-B1l Gli-A3c.

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