Inheritance of signs of resistance to osmotic stresses in genetically modified wheat

DOI: https://doi.org/10.7124/FEEO.v34.1637
Keywords: winter wheat, transgenic plants, proline, osmotic resistance, seed generations

Abstract

Aim. To investigate the inheritance of transgenes and the preservation of increased resistance to drought in the seed generations of genetically modified wheat plants with a double-stranded RNA (dlRNA) suppressor of the proline dehydrogenase (pdh) gene. Methods. PCR, determination of free L-proline (Pro) content and yield structure indicators. Results. Under the influence of osmotic stress, T4 wheat plants were selected with elements that form dlRNA suppressor of the pdh gene of wheat, which retain the sign of increased resistance to water deficit. It was found that T4 plants had a higher content of Pro than their original forms under both normal cultivation conditions and water deficit. It is shown that during droughts, biotechnological plants were characterized by higher grain productivity compared to the original genotypes, while under conditions of sufficient moisture provision, the differences in the elements of the yield structure were insignificant. Conclusions. The analysis of seed generations of transgenic wheat showed variability in the manifestation of signs of increased resistance to osmotic stress, which is due to the instability of recombinant DNA in generations. Testing of T4 wheat under the effects of osmotic stress showed the fact of successful selection of plants with introduced elements that form dlRNA suppressor of the pdh gene and cause the sign of increased resistance to water deficit in seed generations.

References

Raza A., Razzaq A., Mehmood S. S., Zou X., Zhang X., Lv Y., Xu J. Impact of climate change on crops adaptation and strategies to tackle its outcome: A review. Plants. 2019. Vol. 8. Р. 34. doi: 10.3390/plants8020034.

Hossain A., Skalicky M., Brestic M., Maitra S., Ashraful Alam M., Syed M. A., Hossain J., Sarkar S., Saha S., Bhadra P., Shankar T., Bhatt R., Kumar C. A., EL Sabagh A., Islam T. Consequences and mitigation strategies of abiotic stresses in wheat (Triticum aestivum L.) under the changing climate. Agronomy. 2021. Vol. 11 (2). Р. 241. doi: 10.3390/agronomy11020241.

Morgun V. V., Dubrovna O. V., Morgun B. V. Modern biotechnologies for stress-resistant wheat plants. Fiziologiya rasteniy i genetika. 2016. Vol. 48 (3). Р. 196–213. doi: 10.15407/frg2016.03.196. [in Ukrainian]

Morgun B. V., Tishchenko E. N. Molecular biotechnology to improve the resistance of cultivated cereals to osmotic stress. Kyiv : Logos, 2014. 219 p. [in Russian]

Sergeeva L. E, Mykhalska S. I, Komisarenko A. G. Modern biotechnologies for increasing plant resistance to osmotic stresses. Kyiv : Kondor, 2019. 160 s. [in Russian]

Mansour M. M. F., Ali E. F. Evaluation of proline functions in saline conditions. Phytochemistry. 2017. Vol. 140. Р. 52–68. doi: 10.1016/j.phytochem.2017.04.016.

Dubrovna O. V., Priadkina G. O., Mykhalska S. I., Komisarenko A. G. Drought-tolerance of transgenic winter wheat with partial suppression of the proline dehydrogenase gene. Regulatory Mechanism sin Biosystems. 2022. Vol. 13 (4). Р. 385–392. doi: 10.15421/022251.

Kolupaev Y. E., Vainer A. A., Yastreb T. O. Proline: physiological functions and regulation of its content in plants under stress conditions. The bulletin of Kharkiv national agrarian university. Ser. Biol. 2014. Vol. 2. (32). Р. 6–22. Retrieved from: https://repo.btu.kharkov.ua//handle/123456789/9047. [in Russian]

Bharathi J., Anandan R., Benjamin L., Muneer S., Prakash M. Recent trends and advances of RNA interference (RNAi) to improve agricultural crops and enhance their resilience to biotic and abiotic stresses. Plant Physiol Biochem. 2023. Vol. 194. Р. 600–618. doi: 10.1016/j.plaphy.2022.11.035.

Dubrovna O. V., Mykhalska S. I., Komisarenko A. G. Use of RNA Interference Technology for Improving Economically Valuable Traits of Cereal Crops. Cytology and Genetics. Vol. 57 (6). Р. 587–610. doi: 10.3103/s0095452723060026.

Tishchenko O. M., Komisarenko A. G., Mykhalska S. I., Sergeeva L. E., Adamenko N. I., Morgun B.V., Kochetov A. V. Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) in vitro and in planta using the LBA4404 strain harboring binary vector pBi2E with dsRNA-suppressor of proline dehydrogenase gene. Cytology and Genetics. 2014. Vol. 48 (4). Р. 19–30. doi: 10.3103/S0095452714040094.

Mykhalska S. I., Komisarenko A. G., Kurchii V. M., Bronnikova L. І. Physiological and biochemical characteristics of genetically modified winter wheat. Factors of experimental evolution of organisms. 2023. Vol. 32. P. 109–114. doi: 10.7124/FEEO.v32.1545.

Dalakouras A., Wassenegger M., Dadami E., Ganopoulos I., Pappas M., Papadopouloua K. Genetically modified organism-free RNA interference: exogenous application of RNA molecules in plants. Plant Physiology January. 2020. Vol. 182 (1). P. 38–50. doi: 10.1104/pp.19.00570.

Abiri R., Valdiani A., Maziah M., Shaharuddin N. A., Sahebi M., Yusof Z. N. B., Atabaki N., Talei D. A critical review of the concept of transgenic plants: Insights into pharmaceutical biotechnology and molecular farming. Curr Issues Mol Biol. 2016. Vol. 18. P. 21–42.