Androgenesis in vitro in anther culture of bread winter wheat

  • I. S. Zambriborshch Plant Breeding&Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road, 3 https://orcid.org/0000-0003-2430-3690
  • O. L. Shestopal Plant Breeding&Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road, 3 https://orcid.org/0000-0002-2987-9712
  • M. S. Chekalova Plant Breeding&Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road, 3 https://orcid.org/0000-0001-7505-8459
  • O. A. Afinogenov Plant Breeding&Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road, 3 https://orcid.org/0009-0000-9392-0233
  • M. A. Lytvynenko Plant Breeding&Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road, 3
  • O. A. Vasiliev Plant Breeding&Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road, 3
DOI: https://doi.org/10.7124/FEEO.v34.1632
Keywords: dihaploids, wheat, androgenesis in vitro, callus, regeneration

Abstract

Aim. Study of the effectiveness of stages of in vitro androgenesis in anther culture for the creation of dihaploid lines of bread winter wheat. Methods. In vitro culture of isolated wheat anthers. For each genotype, the following parameters of androgenesis in vitro were determined: the percentage of caluses and the percentage of regeneration of green plants from the number of planted anthers; the percentage of acclimatized plants from regenerated green plantlets, as well as the percentage of fertile (doubled haploid) plants. Results. Androgenesis in vitro in the anther culture of 105 genotypes of soft winter wheat of different genetic origin was studied. Differences regarding the frequency of callusogenesis induction (from 0.17 to 21.25 percent of planted anthers) and the ability to regenerate plants (from 0 to 4.94 percent of planted anthers) were found. The dihaploid lines in anther culturefor almost 50 % of the studied genotypes were obtained. Spontaneous diploidization averaged 29.41 % of the obtained regenerants. Conclusions. Genotype-specific morphogenetic reactions of bread winter wheat microspores in the process of in vitro androgenesis were revealed. 122 dihaploid lines of bread winter wheat were obtained.

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