Investigation of the influence of ivermectin on potato plants in vitro
Abstract
Aim. Investigation of the influence of ivermectin dissolved in different organic solvents (DMSO and ethanol) on morpho-physiological parameters of potato plants (Solanum lycopersicum) in vitro. Methods. Potato plants of cv. Vernisage were cultivated in vitro on MS medium in the presence of 10, 50 and 100 μg/ml of ivermectin dissolved in DMSO or ethanol, control plants – on MS medium without addition of any agents, and the plants grown on MS medium supplemented with 0.04, 0.2, 0.4 % of ethanol or DMSO were used as negative control. The influence of ivermectin, DMSO and ethanol was evaluated after 1 month of cultivation by the length of plant shoots, total leaf area and photosynthetic pigments content. Results. The growth-stimulating activity of ivermectin at concentration of 10 μg/ml and of the solvents at concentration of 0.04 % was identified, and also the phytotoxic influence of DMSO at concentrations 0.2 and 0.4 % alone or in the presence of ivermectin dissolved in it was shown in this study. Also, it was found that photosynthetic pigments content in the presence of ivermectin dissolved in ethanol, and in the presence of 0.04 % of ethanol was higher than in control. Conclusions. It has been established that the influence of ivermectin on morpho-physiological parameters of plants depends on the solvent in which it is dissolved for research purposes.
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