Design of potential Ilp markers for Hordeum vulgare and H. marinum Atg2, Atg7, Atg8 genes

DOI: https://doi.org/10.7124/FEEO.v34.1623
Keywords: salt stress, Hordeum marinum, Hordeum vulgare, ILP markers, ATG, autophagy

Abstract

Aim. Design of potential intron length polymorphism markers for ATG2, ATG7, ATG8 genes. Methods. Use of classical bioinformatics methods to search for loci encoding ATG2, ATG7, ATG8 in the Hordeum marinum genome based on homology with cultivated barley genes; analysis of exon-intron structure of genes, design of ILP markers. Results. The sequences of ATG2, ATG7, ATG8 genes in H. marinum were determined. Analyzed differences in the exon-intron structure of the ATG2, ATG7, ATG8 loci in cultivated (H. vulgare) and salt-resistant wild barley (H. marinum). 25 potential ILP markers were designed for introns that showed significant interspecies length polymorphism: 13 for ATG2, 6 for ATG7, 3 for ATG8A, 2 for ATG8B and 1 for ATG8D. Conclusions. Based on gene structure comparative analysis of the autophagosome formation proteins ATG2, ATG7, ATG8 of cultivated H. vulgare and salt-tolerasnt H. marinum, a number of introns are recommended for further use as a potential source of ILP markers for the study of interspecies genetic differentiation of cereals.

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