Selection of marker systems for determining the allelic composition of high molecular glutenin loci in winter wheat

  • B. V. Morgun Institute of Plant Physiology and Genetics, NAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17; Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo str., 148 https://orcid.org/0000-0001-7041-6894
  • N. V. Sandetska Institute of Plant Physiology and Genetics, NAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17 https://orcid.org/0000-0002-0558-2295
  • O. M. Radchenko Institute of Plant Physiology and Genetics, NAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17 https://orcid.org/0000-0002-3168-923X
  • L. H. Velykozhon Institute of Plant Physiology and Genetics, NAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17; Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo str., 148 https://orcid.org/0000-0002-5935-9363
DOI: https://doi.org/10.7124/FEEO.v34.1621
Keywords: Triticum aestivum L., high molecular weight glutenins, PCR, molecular markers, spare grain proteins

Abstract

Aim. Selection of marker systems and conducting multiplex polymerase chain reactions to determine the allelic composition of the Glu-A1, Glu-B1 and Glu-D1 loci, which determine the high quality of flour in common wheat samples. Methods. Polymerase chain reaction (PCR) using specific primers for the Glu-A1, Glu-B1 and Glu-D1 loci of wheat. Results. PCR analysis was performed to detect the insertion at 43 b. p. in the MAR region, in varieties with the Glu-B1al allele, the presence of an amplicon 563 b. p. long was observed, for varieties that carry other alleles of the locus – an amplicon 520 b. p. long. The results on the Glu-D1 locus showed that for № 2, № 3, № 4, № 7, № 8, № 10, № 11, № 19, № 20, № 21 subunits (5 + 10) were identified. Conclusions. With the help of allele-specific primers, the allelic composition of loci of high molecular weight glutenins was determined in 22 lines wheat. Three alleles Glu-A1a, Glu-A1b, Glu-A1c were found in the Glu-A1 locus; in the Glu-B1 locus, only the Glu-B1al allele was detected, there are two alleles at the Glu-D1 locus: Glu-D1a and Glu-D1d (the Glu-D1a allele predominated). As a result of our research, samples of soft wheat (lines № 2, № 7, № 21) were selected, which can be attributed to genotypes with high baking quality of the flour.

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