5s rDNA intergenic spacer of some species of Cruciata and Chondrophyllae sections and its application in taxonomy of genus Gentiana
Abstract
Aim. The 5S rDNA intergenic spacer (IGS) region is a convenient tool for studying genome evolution and systematics of higher plants. The aim was to investigate the nucleotide sequence of 5S rDNA in some species of the sections Cruciata and Chondrophyllae, as well as to study the peculiarities of this region organisation by comparative analysis with other Gentiana species. Methods. Polymerase chain reaction, cloning, sequencing, bioinformatic analysis. Results. The cloned IGS regions of G. laciniata, G. cruciata and G. dahurica were sequenced and analysed. Typical regulatory elements were found in the regions of terminator and external promoter of 5S rRNA gene. Based on the comparative analysis, two groups of species were distinguished that differ significantly in the organisation of this region. One includes G. asclepiadea and species of the sections Gentiana, Ciminalis and Calathianae, and the other includes species of the sections Cruciata and Pneumonanthe. G. laciniata (sect. Chondrophyllae) differs in the IGS organization from species of both groups. Conclusions. The results of taxon grouping are generally consistent with the genus systematics and indicate that 5S rDNA IGS region can be a convenient tool for studying evolution and systematics of the genus.
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