Cytogenetic changes in irradiated rats under influence of guaninium-zinc chloride complex

  • E. N. Shamilov Institute of Radiation Problems of the Ministry of Science and Education of the Republic of Azerbaijan, Azerbaijan Republic, AZ1143, Baku, B. Vahabzadeh, 9 https://orcid.org/0000-0002-5308-8718
  • A. S. Abdullayev Institute of Radiation Problems of the Ministry of Science and Education of the Republic of Azerbaijan, Azerbaijan Republic, AZ1143, Baku, B. Vahabzadeh, 9 https://orcid.org/0000-0002-6338-4596
  • V. E. Shamilli Institute of Radiation Problems of the Ministry of Science and Education of the Republic of Azerbaijan, Azerbaijan Republic, AZ1143, Baku, B. Vahabzadeh, 9
  • M. F. Farajov Institute of Radiation Problems of the Ministry of Science and Education of the Republic of Azerbaijan, Azerbaijan Republic, AZ1143, Baku, B. Vahabzadeh, 9
  • I. V. Azizov Institute of Molecular Biology and Biotechnology of the Ministry of Science and Education of the Republic of Azerbaijan, Azerbaijan Republic, AZ1073, Baku, Pr. Matbuat, 2A https://orcid.org/0000-0002-5910-3923
  • K. A. Hajiyev Ministry of Health of the Republic of Azerbaijan, Azerbaijan Republic, AZ1073, Baku Health Center, Azadlig ave., 112
  • A. A. Jalilova Ministry of Ecology and Natural Resources, National Hydrometeorological Service, Azerbaijan Republic, AZ 1073 Baku, K. Kazimzade str., 100 (A)
DOI: https://doi.org/10.7124/FEEO.v33.1590
Keywords: gamma irradiation, guanine, guaninium-zinc chloride complex, chromosomal aberrations, abnormal spermatozoa

Abstract

Aim. The main goal of this study was to study the anti-radiation effect of guaninium-zinc chloride complex on cytogenetic changes in irradiated rats. Methods. White rats of the Wistar line with an average weight of 110-125 grams served as the material for the detection of the anti-radiation effect of guaninium-zinc chloride complex. Animals were injected intraperitoneally with solutions of guaninium-zinc chloride complex at a concentration of 40 mg/kg 2 hours before irradiation and were subjected to general uniform gamma irradiation from a 60Co source in the Rukhund-20000 irradiation unit at a dose of 3 and 5 Gy. Results. Cytogenetic analysis of animals showed that with a single injection of guaninium-zinc chloride complex solutions before irradiation, the proportion of chromosomal abnormalities decreased markedly, and reached 2.93 %, and the amount of abnormal spermatozoa markedly decreased. Guaninium-zinc chloride complex softened the damaging effects of ionizing radiation. There was no suppression of cell division. Conclusions. It was found for the first time that, with the introduction of a solution of guaninium-zinc chloride complex in white Wistar rats before irradiation, it reduces the damaging effect of gamma irradiation. The obtained data gives grounds to assume that the guaninium-zinc chloride complex tested by us can be recommended as a promising anti-radiation agent.

References

Mamedov N. A., Craker L. E., Shamilov E. N., Rzayev A. A., Abdullaev A. S., Rzayeva I. A., Gasimova N. I., Guliev G. N. Radioprotective Activity Of Some Medicinal Plant Extracts. Science and Horticulture for People. 28th International Horticultural Congress : abstracts (August 22–27, 2010). Lisboa : Lisbon Congress Centre, 2010. Vol. 1. P. 84. Retrieved from: https://www.actahort.org/books/925/925_46.htm. doi: 10.17660/ActaHortic.2011.925.46.

Rzaev A. A., Shamilov E. N., Abdullayev A. S., Kasumova N. I., Kuliev Q. N., Mamedrzaeva E. T., Gaziev A. G., Azizov I. V. Tsitoqenetichicheskiye izmeneniya obluchyonnix kris pod vozdeystviyem rastitelnix biokompleksov. Izvesitiya Akademii Nauk Qruzii, Bioloqicheskaya seriya A. Tbilisi, 2009. Vol. 35. P. 393–401. [in Russian]

Gahramanova S. I., Jalaladdinov F. F., Munshieva M. K., Khudaverdiev R. A., Hamidov R. H., Muradkhanov R. M., Abdullayev A. S., Shamilov E. N., Azizov I. V., Gahramanov T. O. Synthesis and Investigation of Complex Compounds of Divalent Manganese, Copper, Cobalt and Zinc with Tryptophan and their Biological Activity. Int J Chem Sci. 2018. 16 (3). P. 286. doi: 10.21767/0972-768X.1000286.

Asadullina N. R., Gudkov S. V., Bruskov V. I. Antioxidantniye svoystva nekotorix purinovix soedineniy. VIII Mejdunarodnoy konferensii "Bioantioxidant" : tezisi dokladov. 2010. P. 28. [in Russian]

Syoichi Shirotake, Takeichi Sakacushi Complexes formed by guanine or cytosine, and Zn (II). Chem. Pharm. Bull. 1978. 26 (10). P. 2941–2951.

Meredith R. A simple method for preparing meiotic chronosome. Chromosoma. 1969/ Vol. 26. P. 254–258. doi: 10.1007/BF00326520.

Williams D., Hagen A., Runyan J., Lafferty D. A method for the differentiation of male meiotic chromosome stages. J. Hered. 1971. Vol. 62. P. 17–22.

Ford C. E., Hamerton J. L. A colchicines hypotonic citrate squash sequence for mammalian chromosomes. Stain Technol. 1956. Vol. 31. P. 247–251. doi: 10.3109/10520296309061189.

Radjabli S. I., Kryukova E. Sravnitelniy analiz differensialnoy okraski xromosom dvux vidov chomyachkov, daurskoqo i kitayskoqo. Tsitologiya. 1973. Vol. 15. P. 1527–1531. [in Russian]

Sumner A. T. A simple technique for demonstrating centromeric heterochromatin// Exp. Cell Res. 1972. Vol. 75. P. 305–306. doi: 10.1016/0014-4827(72)90558-7.

Howell W. M., Black D. A. Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: in a one step method. Experientia. 1980. Vol. 36. P. 1014–1015. doi: 10.1007/BF01953855.

Bochkov N. P., Kozlov V. M., Sevankaev A. V., Antoshina M. M. Analiz aneuploidii v kulturax embrionalnix fibroblastov cheloveka. Genetika. 1966. No. 10. P. 120–124. [in Russian]

Zakharov A. F. Khromosomi cheloveka. Problemi lineynoy organizatsii. Iz-vo “Medicine”, 1977. 192 p. [in Russian]

Kolomiets O. L., Mazurova T. F., Pomerantseva M. D. Elektronno-mikroskopicheskiy analiz sinaptonemnix compleksov samtsov laboratornix mishey, exponirovannix v period embriogeneza v rayone Chernobilskoy AES. Genetica. 1992. Vol. 28(9). P. 49–57. [in Russian]

Nadzhafova R. S., Bulatova N. Sh., Kozlovskiy A. I., Ryabov I. N. Identifikatsiya strukturnoy perestroyki v kariotipe polevki-ekonomki iz Chernobylya methodami differensionalnoy okraski chromosom. Genetika. 1994. Vol. 30(3). P. 361–366. [in Russian]

Pomerantseva M. D., Chekhovich A. V., Ramayya L. K. Geneticheskiye effekti u mishey, exponirovannix v 10-kilometrovoy zone Chernobylskoy AES. Genetika. 1990. Vol. 26(10). P. 1870–1875. [in Russian]

Pomerantseva M. D., Shevchenko V. A., Ramayya D. K., Testov B. V. Geneticheskiye povrejdeniya u mishey, obitayushix v usloviyax povishennoqo fona radiatsii. Genetika. 1990. Vol. 26(13). P. 466–473. [in Russian]