Analysis of KIR genotyping results in women with recurrent implantation failure

  • K. O. Sosnina State Institution «Institute of Hereditary Pathology of the National, Academy of Medical Sciences of Ukraine», Ukraine, 79008, Lviv, M. Lysenko str., 31a https://orcid.org/0000-0003-4527-2010
  • D. V. Zastavna State Institution «Institute of Hereditary Pathology of the National, Academy of Medical Sciences of Ukraine», Ukraine, 79008, Lviv, M. Lysenko str., 31a https://orcid.org/0000-0002-3858-7180
  • O. I. Terpyliak State Institution «Institute of Hereditary Pathology of the National, Academy of Medical Sciences of Ukraine», Ukraine, 79008, Lviv, M. Lysenko str., 31a https://orcid.org/0000-0001-6274-8362
DOI: https://doi.org/10.7124/FEEO.v33.1572
Keywords: KIR genotyping, Recurrent Implantation Failure (RIF), in vitro fertilization (IVF)

Abstract

Aim. To analyze the frequency and distribution of KIR genotypes in women with recurrent implantation failure after cycles of in vitro fertilization. Methods. DNA extraction and purification, PCR-SSP, agarose gel electrophoresis. Results. The spectrum of KIR genes and the frequency of KIR genotypes in women with recurrent implantation failure were determined. The study group was divided according to history, in particular, a group of 84 women whose diagnosis was outlined by the concept of "repeated failed implantations" was singled out. Statistical analysis of the obtained results using the Pearson test (χ2) indicates a significantly lower frequency of the KIR-AB genotype (χ2 = 9.7; p < 0.005) and a significantly higher frequency of the KIR-AA genotype (χ2 = 7.35; p < 0.01) in women with repeated failed implantations compared to the general group of women with reproductive failures. Conclusions. Taking into account the results of statistical data processing (OR = 2.05; CI 95 %: 1.21-3.45), we consider the KIR-AA genotype to be a significant risk factor for recurrent implantation failure after IVF. We define KIR genotyping as a genetic test to assess the risk of the embryo being rejected by the maternal immune system, and thus to direct medical interventions in order to achieve a successful pregnancy.

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