Alleles at the Gli-A3 locus in groups of ukrainian winter common wheat cultivars of different origin

  • N. O. Kozub Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0000-0002-3572-1786
  • I. O. Sozinov nstitute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33 https://orcid.org/0000-0002-3621-5746
  • H. Ya. Bidnyk Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а
  • N. A. Demianova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а
  • O. I. Sozinova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskogo str., 2а https://orcid.org/0000-0002-0981-3433
  • Y. B. Blume Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2А https://orcid.org/0000-0001-7078-7548
DOI: https://doi.org/10.7124/FEEO.v33.1561
Keywords: Triticum aestivum, gliadin, diversity, minor gliadin locus, climate change

Abstract

Aim. The aim of the study was to analyze the diversity and frequencies of alleles at the minor gliadin locus Gli-A3 in groups of common winter wheat cultivars developed in different breeding institutions of Ukraine in different periods of time. Methods. Acid polyacrylamide gel electrophoresis of gliadins was carried out. To identify the Gli-A3 allele of the cultivar Myronivska storichna, segregation in a sample of F2 seeds from crossing with the cultivar Bezosta 1 was analyzed. Results. Alleles at Gli-A3 were identified in 511 Ukrainian cultivars. In addition to the alleles a-d, a new allele Gli-A3e encoding two omega-gliadins was identified in Myronivska storichna. The alleles a and b predominate in the total group of cultivars. Differences in the allele composition and frequencies were revealed between some groups of cultivars. A significant reduction in the frequency of Gli-A3b was detected for the groups of the Plant Breeding and Genetics Institute (PBGI) and the V. Ya. Yuryev Plant Production Institute (IPP) cultivars released after 2010. Conclusions. The frequencies of the predominant alleles a and b are similar in the groups of the Central Forest-Steppe cultivars in different periods of breeding. An increase in the frequency of the allele a was noted for the groups of PBGI (the Steppe) and IPP (the Eastern Forest-Steppe) cultivars, which may indicate its adaptive value under conditions of an increase in the average annual temperature in these zones.

References

Shewry P. What is gluten – why is it special? Frontiers in Nutrition. 2019. Vol. 6. e. 101. doi: 10.3389/fnut.2019.00101.

Pronin D., Börner A., Weber H., Scherf K. A. Wheat (Triticum aestivum L.) breeding from 1891 to 2010 contributed to increasing yield and glutenin contents but decreasing protein and gliadin contents. J. Agric. Food. Chem. 2020. Vol. 68 (46). P. 13247–13256. doi: 10.1021/acs.jafc.0c02815.

Masci S., Lafiandra D., Porceddu E., Lew E. J. L., Tao H. P., Kasarda D. D. D-glutenin subunits: N-terminal sequences and evidence for the presence of cysteine. Cereal Chemistry. 1993. Vol. 70. P. 581–585.

Metakovsky E. V., Chernakov V. M., Upelniek V. P., Redaelli R., Dardevet M., Branlard G., Pogna N. E. Recombination mapping of omega-gliadin-coding loci on chromosome 1A of common wheat: A revision. J. Genet. Breed. 1996. Vol. 50. P. 277–286.

McIntosh R. A. Catalogue of Gene Symbols. Gene Catalogue. 2013. Retrieved from: http: www.shigen.nig.ac.jp/wheat/komugi/genes/download.jspMacGene.

Metakovsky E., Melnik V., Rodriguez-Quijano M., Upelniek V., Carrillo J. M. A catalog of gliadin alleles: Polymorphism of 20th-century common wheat germplasm. The Crop Journal. 2018. Vol. 6 (6). P. 628–641. doi: 10.1016/j.cj.2018.02.003.

Rodriguez-Quijano M., Carrillo J. M. Linkage map of prolamin loci Gli-D4 and Gli-D5 in hexaploid wheat. Plant Breeding. 1996. Vol. 115. P. 189–191. doi: 10.1111/j.1439-0523.1996.tb00899.x.

Sobko T. A. Identification of a locus controlling synthesis of alcohol-soluble proteins of winter wheat endosperm, Visnyk Silsko-hospodarskoi Nauki. 1984. No. 7. P. 78–80. [in Ukrainian]

Sozinov A., Sozinov I., Kozub N., Sobko T. Stable gene associations in breeding and evolution of grasses. Evolutionary Theory and Processes: Modern Perspectives. Papers in Honor of Eviatar Nevo. Wasser S. P. (ed.). Kluwer Academic Publishers, 1999. P. 97–113. doi: 10.1007/978-94-011-4830-6_7.

Kozub N. A., Sozinov I. A., Sobko T. A., Kolyuchii V. T., Kuptsov S. V., Sozinov A. A. Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine. Cytol. Genet. 2009. Vol. 43 (1). P. 55–62. doi: 10.3103/S0095452717020050.

Kozub N. A., Sozinov I. A., Karelov A. V., Blume Ya. B., Sozinov A. A. Diversity of Ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes. Cytol Genet. 2017. Vol. 51 (2). P. 117–129. doi: 10.3103/S0095452717020050.

Kozub N. O., Sozinov I. O., Chaika V. M., Sozinova O. I., Janse L. A., Blume Ya. B. Changes in allele frequencies at storage proteins of winter common wheat under climate change. Cytol Genet. 2020. Vol. 54 (4). P. 305–317. doi: 10.3103/S0095452720040076.

Perrier X., Jacquemoud-Collet J. P. DARwin Software. 2006. Retrieved from: http://darwin.cirad.fr/.

Quraishi U. M., Pont C., Ain Q. U., Flores R., Burlot L., Alaux M., Quesneville H., Salse J. Combined genomic and genetic data integration of major agronomical traits in bread wheat (Triticum aestivum L.). Front Plant Sci. 2017. Vol. 8. e1843. doi: 10.3389/fpls.2017.01843.

Soriano J. M., Alvaro F. Discovering consensus genomic regions in wheat for root-related traits by QTL meta-analysis. Sci. Rep. 2019. Vol. 9. e10537. doi: 10.1038/s41598-019-47038-2.