Physiological and biochemical characteristics of genetically modified winter wheat

Keywords: Triticum aestivum L., proline, proline dehydrogenase, carbohydrates, sucrose, fructose


Aim. Analysis of physiological and biochemical characteristics of genetically modified wheat with partially suppressed activity of the proline dehydrogenase gene. Methods. Determination of proline dehydrogenase (PDH) activity, free L-proline (Pro); carbohydrate content (sucrose and fructose). Results. It was established that under conditions of water deficit in genetically modified wheat plants with integrated elements forming the double-stranded RNA suppressor of the pdh gene, there is a partial inhibition of the enzyme activity and an increase in the content of free proline. Changes in carbohydrate metabolism under water deficit stress and in the first hours after rehydration were noted, while the sucrose / fructose ratio in control plants significantly decreases during dehydration and normalizes when water supply is restored, in genetically modified plants this indicator almost does not change under short-term water deficit stress. Conclusions. Partial suppression of the proline dehydrogenase gene leads to an increase in the free proline content, the fluctuations of which contribute to the maintenance of carbohydrate balance.


Shrawat A. K., Armstrong C. L. Development and application of genetic engineering for wheat improvement. Critical Reviews in Plant Sciences. 2018. Vol. 37 (5). P. 335–421. doi: 10.1080/07352689/2018/1514718.

Servet C., Ghelis T., Richard L., Zilberstein A., Savoure A. Proline dehydrogenase: A key enzyme in controlling cellular homeostasis. Frontiers in Bioscience – Landmark. 2012. Vol. 17 (2). P. 607–620. doi: 10.2741/3947.

Hossain A., Skalicky M., Brestic M., Maitra S., Ashraful Alam M., Syed M.A., Hossain J., Sarkar S., Saha S., Bhadra P., Shankar T., Bhatt R., Kumar C. A., EL Sabagh A., Islam T. Consequences and mitigation strategies of abiotic stresses in wheat (Triticum aestivum L.) under the changing climate. Agronomy. 2021. Vol. 11 (2). Р. 241. doi: 10.3390/agronomy11020241.

Sarker U., Oba S. The response of salinity stress-induced A. tricolor to growth, anatomy, physiology, non-enzymatic and enzymatic antioxidants. Front Plant Sci. 2020. Vol. 11. P. 1354. doi: 10.3389/fpls.2020.559876.

Kolupaev Yu. E., Vainer A. A., Yastreb T. O. Proline: physiological functions and regulation of the content in plants under stress conditions Newsletter. Kharkiv. nat. agrarian. un-tu. Ser. Biol. 2014. Vol. 2. P. 6–22. [in Russian]

Carvalho K., Campos M. K., Domingues D. S. The accumulation of endogenous proline induces changes in gene expression of several antioxidant enzymes in leaves of transgenic Swingle citrumelo. Molecular Biology Reports. 2013. Vol. 40. P. 3269–3279. doi: 10.1007/s11033-012-2402-5.

Major P. S., Zakharova V. P., Velikozhon L. G. Investigatio de cumulatione proline et saccharo in siligineis genotypes differentibus in gradu resistentiae pruinae. Res gestae et quaestiones geneticae, generandi et biotechnologiae. 2007. Vol. 1. P. 121–128. [in Ukrainian]

Komisarenko A. G., Mykhalskaya S. I., Kurchiy V. M. Investigation of transgene functionality in T2 biotechnological plants of winter wheat on the basis of osmostability. Factors of experimental evolution of organisms. 2021. Vol. 28. P. 88–93. doi: 10. 7124/FEEO.v28.1381. [in Ukrainian]

Andriushchenko V. K., Sayanova V. V., Zhuchenko A. A., Diyachenko N. I., Chilikina L. A., Drozdov V. V., Korochkina S. K., Cherep G. I., Medvedev V. V., Niutin Yu. I. The modification of proline estimation method for detection drought tolerant forms of genus Lycopersicon Tourn. Izv. Akad. Nauk Mold. SSR. 1981. Vol. 4. P. 55–60. [in Russian]

Mattioni C., Lacerenza N. G., Troccoli A., de Leonardis A. M., di Fonzo N. Water and salt stress-induced alterations in proline metabolism of Triticum durum seedlings. Physiol. Plant. 1997. Vol. 101. P. 787–792. doi: 10.1111/j.1399-3054.1997.tb01064.x.

Sakalo V. D., Larchenko K. A., Kurchii V. M. Synthesis and metabolism of sucrose in leaves of corn seedlings under conditions of water deficit. Physiology and biochemistry cult. plants. 2009. Vol. 41 (4). P. 305–313. [in Ukrainian]

Kolupaev Yu. E., Karpets Yu. V. Formation of adaptive reactions of plants to the action of abiotic stressors. Kiev : Basis, 2010. 352 p.

Sergeeva L. E., Mykhalska S. I., Komisarenko A. G. Modern biotechnologies for increasing plant resistance to osmotic stresses. Kyiv : Kondor, 2019. 160 p. [in Russian]

Proels R. K., Hückelhoven R. Cell-wall invertases, key enzymes in the modulation of plant metabolism during defence responses. Mol. Plant Pathol. 2014. Vol. 15 (8). P. 858–864. doi: 10.1111/mpp.12139.