Protective effect of zinc complex with hypoxanthine-9-riboside on wheat seedlings grown from gamma-irradiated seeds

E. N. Shamilov; A. S. Abdullayev; V. E. Shamilli; I. V. Azizov

doi:10.7124/FEEO.v31.1504

E. N. Shamilov Institute of Radiation Problems of ANAS, AZ1143, Azerbaijan Republic, Baku, B. Vahabzade, 9 https://orcid.org/0000-0002-5308-8718
A. S. Abdullayev Institute of Radiation Problems of ANAS, AZ1143, Azerbaijan Republic, Baku, B. Vahabzade, 9 https://orcid.org/0000-0002-6338-4596
V. E. Shamilli Institute of Radiation Problems of ANAS, AZ1143, Azerbaijan Republic, Baku, B. Vahabzade, 9
I. V. Azizov Institute of Molecular Biology and Biotechnology of NAS of Azerbaijan, Azerbaijan, AZ1073, Baku, Pr. Matbuat, 2A https://orcid.org/0000-0002-5910-3923

DOI: https://doi.org/10.7124/FEEO.v31.1504

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Keywords: zinc complex, hypoxanthine-9-riboside, gamma irradiation, chromosomal aberrations, chlorophylls, carotenoids

Abstract

Aim. The aim of the research was to obtain the zinc complex hypoxanthine-9-riboside and to study its effect during γ-irradiation on the biosynthesis of chlorophylls, carotenoids and on the release of chromosome aberrations in anaphase root hair cells in wheat seedlings. Methods. The zinc complex was obtained by direct interaction of zinc chloride – ZnCl₂ with hypoxanthine-9-riboside. X-ray phase analysis and thermogravimetric measurements of the obtained complex were carried out. Before irradiation, seeds of durum wheat Triticum durum L. from a ⁶⁰Co source were treated with a zinc complex with hypoxanthine-9-riboside at concentrations of 0.1; 0.01; 0.001%. Structural changes in chromosomes were determined in the initial and final stages of anaphase. Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.

References

Grodzinsky D.M. Radiobiology of plants. Kiev: Naukova Dumka, 1989. 380.

Lichtenthaler H.K., Wellburn A.R. Chlorophylls and carotenoids: pigments of photosynthetic biomembrane. Methods Enzymol. 1987. 148. P. 350–382.

Udovenko G.V., Goncharova E.A. Influence of extreme environmental conditions on the structure of the crop of agricultural plants. Methodical instructions. Moskva: Gidrometeoizdat, 1982. 144 p.

Siedlecka A., Krupa Z. Interaction between cadmium and iron and its effects on photosynthetic capacity of primary leaves of Phaseolus vulgaris. Plant Physiol. Biochem. 1996. Vol. 35. P. 951–957.

Gushcha N.I., Perkovskaya G.Yu., Dmitriev A.P., Grodzinsky D.M. Influence of chronic irradiation on the adaptive potential of plants. Radiation biology. Radioecology. 2002. Vol. 42(2). P. 155–158.

Zhuravskaya A.N., Voronov I.V., Poskachina E.R., Sleptsov I.V. Influence of gamma irradiation and lyophilizate of amaranth anterosa on photosynthesis of wheat seedlings. Science and Education. 2015. No. 3. P. 76–83.

Tishkevich T.K., Petrovich I.S., Zabolotny A.I. Changes in the physiological and biochemical characteristics of lupine plants under the influence of incorporated radionuclides. Biology. 1993. Vol. 33. P. 54–57.

Grigoreva A.S., Konakhovich N.F., Kriss E.E., Maletin Yu.A. Interaction between triphenylverdazyl radical and complexes of copper, iron, aluminum and zinc with N-3-trifluoromethylphenylanthranilic acid. Coordination chemistry. 1981. Vol. 11(12). P. 1620–1625.

Azizov I., Shamilov E., Abdullayev A., Muslimova Z., Mamedli G. Influence of a Modified Plant Extract on Activity of Antioxi-dant Enzymes and Concentration of Pigments in Gamma-Irradiated Plants of Maize and Wheat. Proceedings of the Latvian Acad-emy of Sciences. Section B. Natural, Exact and Applied Sciences. 2018. Vol. 72(1). P. 38–42.

Abdullayev A.S., Shamilov E.N., Farajov M.F., Azizov I.V. The Radioprotective Properties of Imatinib Mesylate-Zinc Complex in Plants. Journal of Advanced Biotechnology and Bioengineering. 2021. 9. P. 17–22.

Shamilov E.N., Abdullayev A.S., Shamilli V.E, Asgerova T.Y., Gahramanova Sh. I., Jalaladdinov F. F. Protective properties of the nickel (II) complex with tryptophan. Factors of experimental evolution of organisms. 2021. Vol. 29. P. 191–195. doi: 10.7124/FEEO.v29.1430

Shamilov E.N., Abdullayev A.S., Farajov M.F. Study of the radioprotective properties of the Copper (II) comlex with triptophan in wheat seedlings. Internetaional conference on experimental sciences and biotexnology. Turkey, 2021. 52 p.

Shlyk A.A. Determination of chlorophyll and carotenoids in green leaf extracts. In book. Biochemical methods in plant physiology. Ed. Polyanova O.M. Moskva: Nauka, 1971. P. 154–171.

Harri Ldnnberg and Paula Vihanto. Complexing of Inosine and Guanosine with Divalent Metal Ions in Aqueous Solution. Inorganica Chimica Acta. 1981. 56. P. 157–161.

Martin R.B., Mariam Y.H. In ‘Metal Ions in Biological Systems’. 1979. Vol. 8, H. Sigel, ed., Marcel Dekker, New York. P. 57–126.