Genetic analysis of palmytic acid content in the oil of maize based on Waxy mutation
Abstract
Aim. Determination of the effect of wx mutation on the palmitate content and genetic analysis of this trait in waxy maize. Methods. In two-year experiments, 10 unrelated maize inbreds of the common type and 10 inbreds – carriers of the wx mutation were analyzed. Genetic analysis of palmitate content was carried out in the diallel crosses of 6 indreds – carriers of the wx mutation by the second Griffing method. The palmitate content was determined by Peysker’s gas chromatographic method. Results. Carriers of the wx mutation of maize exceeded the maize of common type in the content of glycerides of palmitic acid in oils. The content of palmitate in unrelated inbreds – carriers of the wx mutation was subjected to quantitative variability and ranged from 13.3 % to 16.4 %. Inheritance of palmitate content in the waxy corn was carrired out by the type of incomplete dominance with a predominant contribution of additive effects to the variation. A higher level of trait was controlled by recessive alleles of polygens. Conclusions. The obtained results suggest the presence in chromosome 9 of spatial linkage of the mutant wx gene with palmitate - coding locus, the effect of which is modified by a polygenic complex.
References
Scrimgeour C. Chemistry of fatty acids. Bailey’s industrial oil and fat products : monograph/ F.Shahidi Ed.-Hoboken, New Jersey : J. Wiley & Sons Inc. Publ., 2005. Vol. 1. Ch. 1. P. 1–44. doi: 10.1002/047167849X.bio005.
Bates P.D., Stymme S., Ohlrogge J. Biochemical pathways in seed oil synthesis. Curr.Opin.Plant Biol. 2013. Vol. 16 (3). P. 358–364. doi: 10.1016/j.pbi.2013.02.015.
Fattore E., Fanelli R. Palm oil and palmitic acid : a review on cardiovascular effects and carcinogenicity. Int. J. Food Sci. Nutr. 2013. Vol. 64 (5). P. 648–659. doi: 10.3109/09637486.2013. 768213.
Dhaka V., Gulia N., Ahlawat K.S., Khatkar B.S. Trans fats – sources, health risks and alternative approach : a review. J Food Sci. Technol. 2011. Vol. 48 (5). P. 534–541. doi: 10.1007/s13197-010-0225-8.
Cook J.P., McMullen M.D., Holland J.B., Tian F., Bradbury P., Ross-Ibarra J., Buckler E.S., Flint-Garcia S.A. Genetic architecture of maize kernel composition in the nested association mapping and inbred association panels. Plant Physiol. 2012. Vol. 158 (2). P. 824–834. doi: 10.1104/pp.111.185033.
Sanjeev P., Chaudhary D.P., Sreevastava P., Saha S., Rajendran A., Sekhar J.C., Chikkappa G.K. Comparison of fatty acid profile of specialty maize to normal maize. J. Amer. Oil Chem. Soc. 2014. Vol. 91 (6). P. 1001–1005. doi: 10.1007/s11746-014-2429-y.
Yang X, Guo Y., Yan J., Zhang J., Song T., Rocheford T., Li J.-S. Major and minor QTL and epistasis contribute to fatty acid compositions and oil concentration in high-oil maize. Theor. Appl.Genet. 2010. Vol. 120. P. 665–678. doi: 10.1007/s00122-009-1184-1.
Coe E.H., Schaeffer M.L. Genetic, physical maps and database resources for maize. Maydica. 2005. Vol. 50 (3). P. 285–303.
Dospekhov B.A. Technics of field experiment (with the basics of statistical processing of research results). 6th Ed. Moscow : Alliance, 2011. 350 p. [in Russian]
Gutsol V.V., Zhuravel I.A., Guryeva I.G., Kislichenko, V.S. The study of fatty acids in the seeds of lettuce variety «Lollo Rosso». Bull. Kazakh Med.Univ. 2015. Vol. 4. P. 476–478. [in Russian]
Litun P.P., Proskurnin N.V. Genetics of quantitative traits : genetic crossings and genetic analysis. Kyiv : UMVO, 1992. 96 p. [in Russian]
Vukolov E.A. Foundations of statistical analysis. Workshop on statistical methods and operations research using the Statistica and Exel Packages : Tutorial. 2nd Ed. Moscow: Forum. 2008. 464 p. [in Russian]
Shannon J.C., Garwood D.L., Boyer C.D. Genetics and physiology of starch development. Starch chemistry and technology : monograph/ J.BeMiller, R.Whistler Eds. New-York : Acad.Press. 3rd Ed. 2009. Cpt. 3. P. 23–82. doi: 10.1016/B978-0-12-746275-2.-00003-3.
Wassom J.J., Mikkelineni V., Bohn M.O., Rocheford T.R. QTL for fatty acid composition of maize kernel oil in Illinois High Oil × B73 backcross-derived lines. Crop Sci. 2008. Vol. 48 (1). P. 69–78. doi: 10.2135/cropsci2007.04.0208.
Orhun G.E. Genetic control of oil and saturated fatty acids in maize (Zea mays L.) populations. J. Agr. Fac. Gas. Univ. 2018. Vol. 35 (3). P. 242–247. doi: 10.13002/jafag4388.
