Common wheat genotypes with null-alleles at gliadin loci

  • N. O. Kozub Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Osypovskogo str., 2а https://orcid.org/0000-0002-3572-1786
  • I. O. Sozinov Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33 https://orcid.org/0000-0002-3621-5746
  • H. Ya. Bidnyk Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Osypovskogo str., 2а
  • N. A. Demianova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Osypovskogo str., 2а
  • O. I. Sozinova Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Osypovskogo str., 2а https://orcid.org/0000-0002-0981-3433
  • A. V. Karelov Institute of Plant Protection, NAAS, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Osypovskogo str., 2а https://orcid.org/0000-0001-6548-2504
  • S. I. Spivak Institute of Food Biotechnology and Genomics, NAS of Ukraine, Ukraine, 04123, Kyiv, Osypovskogo str., 2а https://orcid.org/0000-0002-1928-2744
  • Ya. B. Blume ДУ «Інститут харчової біотехнології і геноміки НАН України», Україна, 04123, м. Київ, вул. Осиповського, 2а https://orcid.org/0000-0001-7078-7548
DOI: https://doi.org/10.7124/FEEO.v31.1485
Keywords: bread wheat, omega-5 gliadin, omega-1,2 gliadin, allergy, null-allele

Abstract

Aim. The study was aimed at identification and selection of winter common wheat genotypes with null-alleles at the gliadin loci Gli-B1 and Gli-D1. Methods. The search for spontaneous mutants with the absence of synthesis of gliadin blocks was made in winter common wheat hybrid material and cultivars. To identify mutations, APAG electrophoresis and SDS-electrophoresis of seed storage proteins were performed. Results. The frequency of spontaneous mutations resulting in the null-allele at the loci Gli-B1 and Gli-D1 in F2 was 0,1 % and 0,05 %, respectively, in the cross Odesska chervonokolosa × B-16. Via sowing the material with those mutations and marker selection, F6 lines were developed: the line OB-Bnull with the null-allele at the Gli-B1 locus and the line OB-Dnull with the null-allele at the Gli-D1 locus. Another line with the null-allele at Gli-B1 was produced from the cross B3 × D4 by marker selection, as well as a biotype with the null-allele at the Gli-D1 locus was selected from the cultivar Slaven. Conclusions. The developed lines with null-alleles at the gliadin locus Gli-B1 (without omega-5 gliadins) and Gli-D1 (without omega-1,2 gliadins) are initial material for hypoallergenic wheat breeding.

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