The peculiarities of plant breeding and drought-resistance mechanisms

  • R. V. Kovbasenko Institute of Cell Biology and Genetic Engineering of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 148 https://orcid.org/0000-0002-0774-362X
  • A. P. Dmitriev Institute of Cell Biology and Genetic Engineering of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 148
  • S. A. Polyakovsky Institute of Cell Biology and Genetic Engineering of Natl. Acad. Sci. of Ukraine, Ukraine, 03680, Kyiv, Akad. Zabolotnogo str., 148
DOI: https://doi.org/10.7124/FEEO.v31.1484
Keywords: tomatoes, in vitro, peroxidase

Abstract

Aim. To propose a plan of cellular selection of tomato plants for resistance to drought with the use of selective factor is manit. Methods. The object of study was the tomato variety Svitanok. Work with cell culture in vitro, aseptic seed germination, microclonal reproduction, callusogenesis and initiation of morphogenesis were performed according to known methods. Results. The influence of peroxidase in the stimulation of protective mechanisms has been established and the role of phytohormones in these processes has been revealed. It was found that when using stepwise selection of tomato cell culture in vitro, samples of TTP-1 and TTP-2, tolerant to salinity, were selected and worked on. The effect of peroxidase on the growth of plant resistance during foliar treatment with signaling molecules is also shown. Conclusions. It has been shown that signaling molecules are actively involved in the antioxidant protection of plants under stress caused by air and soil drought. The signaling molecules we use have a protective effect under stress: specialty characteristic is a very active part in the recovery process after the adverse effects of various factors. The increase in the activity of the antioxidant enzyme peroxidase also contributes to the activation of the systemic resistance of tomato plants against high temperatures.

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