Identification of dehydrin genes Dhn1 and Zmdhn13 alleles in maize varieties and lines

  • M. V. Halaieva Plant Breeding and Genetics Institute - National Center of Seedand Cultivar Investigations of NAAS of Ukraine
  • V. I. Fait Plant Breeding and Genetics Institute - National Center of Seedand Cultivar Investigations of NAAS of Ukraine
DOI: https://doi.org/10.7124/FEEO.v28.1373

Abstract

Aim. Determination of molecular genetic polymorphism of maize varieties and lines (Zea mays L.) of Ukrainian and world selection by Dhn1 and ZmDHN13 dehydrin genes. Methods. Polymerase chain reaction (PCR), PAAgel-electrophoresis. Results. Maize varieties and lines of different geographical origin and different years of creation were analyzed by Dhn1 and ZmDhn13 dehydrin genes. Conclusions. The use of directional primers revealed five different alleles of the Dhn1 locus, which differed in the size of the amplification product: 186, 190, 194, 196 and 200 bp. The most common allele was 196 bp (46.2%). Two alleles of the ZmDhn13 locus were detected: 86 bp and 82 bp. Allele frequency 86 bp was 61.5% and the allele frequency 82 bp was 38.5%. The sequences of the dehydrin genes Dhn1 and ZmDhn13 are not conservative, deletions and / or insertions within these genes are observed. Accordingly, the structure and functional activity of the dehydrin proteins that encode these genes in different genotypes can vary greatly.

Keywords: Zea mays L., dehydrins, genes, drought resistance.

References

Thomashow M.F. Plant cold acclimation: Freezing Tolerance Genes and Regulatory Mechanisms. Annu Rev Plant Physiol Plant Mol Biol. 1999. Vol. 50. P. 571–599. doi: 10.1146/annurev.arplant.50.1.571.

Tunnacliffe A., Wise M.J. The continuing conundrum of the LEA proteins. Naturwissenschaften. 2007. Vol. 94 (10). P. 791–812. doi: 10.1007/s00114-007-0254-y.

Graether S.P., Boddington K.F. Disorder and function: a review of the dehydrin protein family. Frontiers in plant science 2014. Vol. 5. P. 576. doi: 10.3389/fpls.2014.00576.

Liu Y., Liang J.N., Sun L.P., Yang X.H., Li D.Q. Group 3 LEA protein, ZmLEA3, is involved in protection from low temperature stress. Front. Plant Sci. 2016. Vol. 7. P. 1011. doi: 10.3389/fpls.2016.01011.

Liu H., Yu C., Li H., Ouyang B., Wang T., Zhang J., Wang X., Ye Z. Overexpression of ShDHN, a dehydrin gene from Solanum habrochaites enhances tolerance to multiple abiotic stresses in tomato. Plant Sci. 2015. Vol. 231. P. 198–211. doi: 10.1016/j.plantsci.2014.12.006.

Lin C.H., Peng P.H., Ko C.Y., Markhart A.H., Lin T.Y. Characterization of a novel Y2K-type dehydrin VrDhn1 from Vigna radiate. Plant and Cell Physiology. 2012. Vol. 53 (5). P. 930–942. doi: 10.1093/pcp/pcs040.

Hara M., Shinoda Y., Tanaka Y., Kuboi T. DNA binding of citrus dehydrin promoted by zinc ion. Plant Cell Environ. 2009. Vol. 32. P. 532–541. doi: 10.1111/j.1365-3040.2009.01947.x.

Liu Y., Li D., Song Q., Zhang T., Li D., Yang X. The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco. The crop Journal. 2019. Vol. 7. P. 403–410. doi: 10.1016/j.cj.2018.09.001.

Riley A.C., Ashlock D.A., Graether S.P. Evolution of the modular, disordered stress proteins known as dehydrins. PLoS ONE. 2019. 14 (2). e0211813. doi: 10.1371/journal.pone.0211813.

Badicean D., Scholten S., Jacota A. Transcriptional profiling of Zea mays genotypes with different drought tolerances – new perspectives for gene expression markers selection. Maydica. 2011. Vol. 56. P. 61–69.

Liu Y., Wang L., Zhang T., Yang X., Li D. Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress : Scientific Reports. 2017. Vol. 7. P. 1–10. doi: 10.1038/s41598-017-07852-y.