The bystander effect formation in the meristem of Allium cepa L. plants with different genotype

  • O. Yu. German V. N. Karazin Kharkiv National University
  • А. М. Bratchenko V. N. Karazin Kharkiv National University
  • Ye. O. Lytovchenko V. N. Karazin Kharkiv National University
DOI: https://doi.org/10.7124/FEEO.v28.1368

Abstract

Aim. Obtaining and analysis of cytogenetic parameters in meristem cells of Allium cepa L. seedling sprouting from unirradiated seeds, while germinating them with seeds, that were exposed to γ-radiation in different doses. Methods. Cytogenetic analysis included analysis of mitotic activity, anaphase method of chromosome mutations, nucleolar analysis. The F-test was used to compare the control and experimental samples. Results. The formation of the bystander effect is shown in the case of joint germination of irradiated and non-irradiated seeds. The severity of bystander effect increases with increasing irradiation dose. Conclusions. Irradiation of seeds with a dose of gamma radiation of 40 Gy causes the formation of radiation hormesis by the criterion of mitotic activity in the root meristem cells of seedlings, and the irradiation with a dose of 10 Gy does not affect the level of cell proliferation. The bystander effect formation occurs during the joint germination of intact and irradiated seeds as an increase in the level of mitotic activity, and an increase in the number of chromosomal mutations.

Keywords: bystander effect, γ-radiation, meristem, mitotic activity, chromosomal abnormalities, micronuclei.

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