Changeability in the pattern of white marking on a leaf of Trifolium repens L. is a bioindicator of presence in soil of agricultural lands of nitrate nitrogen and heavy metals

  • V. M. Toryanik
  • L. P. Mironets


Aim. Conduct research between the variability of the pattern of white marking on a leaf of Trifolium repens L. and the content of nitrate nitrogen and heavy metals in soil of hayfield and pasture. Methods. Collection of leafs was carried out in 2019 during the period of mass flowering of plant. Identification of drawings of white marking on a leaf was carried out according to the method of IT. Paponova and P.Ya. Schwartzman, using the tables of J. L. Briubecker. Soils were tested for nitrate nitrogen and heavy metals in accordance with recommended methods. Results. In the total sample of T. repens L. collected from territories of the hayfield and pasture, detected 8 phenotypes, formed by a series of 7 alleles of gen V. In the sample from the territory of the hayfield, the phenotype O was most often found, in the sample from the territory of the pasture - the phenotypes of AH and C, much more often than in the territory of the hayfields – the phenotype of the BHC. In the hayfield, the frequency of «wild» phenotypes was higher, in the grassland – «mutant» phenotypes. In each of the studied territories unique phenotypes were detected: on the territory of hayfield – phenotype C in the form of a four-leaf, on the territory of the pasture – phenotypes of BHB and E. Pasture soil had a significantly higher content of nitrate nitrogen (almost 4 times), Fe (almost 2.5 times), Mn (1.6 times), Zn (2.4 times), Cu (1.8 times), Pb (more than 2 times) and Cd (more than 2 times) compared to soil of hayfield. Conclusions. A correlation was found between the frequency of T. repens phenotypes detected in the pattern of white markings on the leave and the presence in soil of hayfield and pasture of nitric nitrogen and heavy metals. However, it should be evaluated with caution, as the relationship between these indicators is although probable, but requires further investigation.

Keywords: Тrifolium repens L., white marking on a leaf, bioindicator, soil, nitrate nitrogen, heavy metals.


Valiev R.R., Yakovleva OM Comparative characteristics of hereditary polymorphism based on «white» marking on leaf of plants in T. repens populations in the city of Ufa and some areas of the Republic of Bashkortostan. Vestn. Bashkir. Un. 2008. V. 13, No. 2. P. 273–276. [in Russian]

Gorshkova T.A. Evaluation of the possibility of using creeping clover (Trifolium repens L.) for bioindication of anthropogenic environmental exposure. News of the Samara Scientific Center of the Russian Academy of Sciences. 2012. Vol. 14, No. 1. P. 69–73. [in Russian]

Levitsky S.N. Genetic polymorphism in the populations of Trifolium repens, growing under conditions of different anthropogenic load areas. Basic research. 2013. No. 4 (pt. 1). P. 108–111. [in Russian]

Sokolova G.G., Kamaltdinova G.T. Morphogenetic polymorphism of clover leaves creeping. News AltGU. 2010. No. 3 (pt. 1). P. 48–51. [in Russian]

Khokh A.N., Lozinskaya OV, Melnov S.B. Environmental quality assessment using meadow clover (Trifolium pratense). Bulletin of Polesye State University. A series of natural sciences. 2011. No. 2. P. 3–7. [in Russian]

Toryanik V.N., Mironets L.P. Morfogenetic polymorphism of T. repens in the pattern of white marking on a leaf on the territory of the city of Sumy with a different anthropogenic load. Faktori eksperimental'noi evolucii organizmiv. 2019. Vol. 25. P. 92–96. doi: 10.7124/FEEO.v.25.1145.

Dospehov B.A. Methods of field experience (with the basics of statistical processing of research results). Moscow: Agropromizdat, 1985. 351 p. [in Russian]

Shvartsman P.Ya. Field practice in genetics with the basics of breeding. Moscow: The Enlightenment, 1986. 111 p. [in Russian]

Brewbaker J. L. V-leaf Markings of White Clover. J. Hered. 1955. Vol. XLVI, No. 3. P. 115–125. doi: 10.1093/oxfordjournals.jhered.a106537

GOST 12071-2014 Grunty. Otbor, upakovka, transportiyrovanie i khranenie obraztsov. [in Russian] Retrieved from:

Dyshko V.N., Dyshko V.V., Romanenko P.V., Studenkova N.V. Methods of agrochemical studies of soils and plants: a training manual. Smolensk: FSBEI HPE «Smolensk State Agricultural Academy», 2014. 197 р. [in Russian]

Yakist' gruntu. Vyznachennia vmistu rukhomykh spoluk marhantsiu (tsynku, kadmiiu, zaliza, kobal'tu, midi, nikeliu, khromu, svyntsiu) v grunti v buferniy amoniyno-atsetatniy vytiazhtsi z рН 4,8 metodom atomno-absorbtsiynoi spektrofotometrii: DSTU 4770.1–9:2007. [in Ukrainian]

Avdeev V.I. Modern methods of biometrics in the study of plants: a textbook. Orenburg: OGAU, 2015. 130 p. [in Russian]

Atramentova L.A., Utevskaya O.M. Biometrics. Part II Group comparison and communication analisis: a textbook. Kharkiv: Ranok, 2007. P. 50–61 [in Ukrainian]

Zhovinsky E.Y., Kuraeva I.V. Geochemistry of heavy metals in soils of Ukraine. Kyiv: Naukova dumka, 2002. 213 p. [in Ukrainian]