Reproduction and endoreduplication in Drosophila melanogaster Meig. when influenced by lead nitrate

  • V. Yu. Strashnyuk
  • O. V. Taglina

Abstract

Aim. The purpose of investigation was to study the reproductive ability and polyteny degree of chromosomes in Drosophila melanogaster Meig. under the influence of various concentrations of lead nitrate. Methods. Canton-S wild-type strain was used as the material. Flies developed on standard sugar-yeast medium, to which in the experiment lead nitrate was added in concentrations of 0.1, 1, and 10 mg/ml. The reproductive ability of the strain was evaluated by the number of adult offspring. The polyteny degree of chromosomes was studied on squashed preparations of larva salivary glands stained with acetoorsein by cytomorphometry. The preparations were obtained at late 3rd instar. Results. The number of adult offsprings decreased when lead nitrate was added to the nutrient medium: at a concentration of 0.1 mg/ml – by 22.8 %, at 1 mg/ml – by 38.9 %. A concentration of 10 mg/ml was lethal. Males showed greater sensitivity to the drug compared to females. The degree of polyteny of chromosomes in the salivary glands of larvae decreased on average by 5.0–6.5 %. Conclusions. Lead nitrate causes a significant, dose-dependent decrease in the reproductive ability of fruit flies and has a toxic effect on Drosophila cells, inhibiting the process of endoreduplication.

Keywords: Drosophila melanogaster Meig., heavy metals, fecundity, giant chromosomes, polyteny.

References

WHO: Lead poisoning and health. Newsroom. Retrieved from: https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health.

Kolosova I.I. Effect of lead acetate, salts of heavy metals on reproduction. Bulletin of Problems in Biology and Medicine. 2013. Vol. 2 (103), Is. 3. P. 13–17. [in Russian]

Nefodova O.O., Zadesenets I.P., Galperin A.I. Influence of cadmium and lead compaunds on morphogenesis of internal organs in ontogenesis. Bulletin of Problems in Biology and Medicine. 2017. Vol. 3 (141), Is. 4. P. 61–66. [in Russian] doi: 10.29254/2077-4214-2017-4-3-141-61-66

Morais S., Costa F.G., Pereira M.L. Heavy metals and human health. In: Environmental Health - Emerging Issues and Practice. 2012. P. 227–246. doi: 10.5772/29869.

Kolosova I.I. Morphological characteristics of rat ovaries at different stages of pregnancy in normal and in condition of lead intoxication. Bulletin of Problems in Biology and Medicine. 2016. Vol. 1 (103), Is. 1. P. 281–287. [in Ukrainian]

Yamasaki T. Measurement of fitness and its components in six laboratory strains of Drosophila melanogaster. Genetics. 1984. Vol. 108. P. 201–211.

Strashnyuk V.Yu., Nepeivoda S.N., Shakhbazov V.G. Cytomorphometric analysis of Drosophila melanogaster Meig. polytene chromosomes in relation to heterosis, selection for adaptively valuable traits, and sex. Rus. Journ. Genet. 1995. Vol. 31. P. 17–21.

Rodman T.C. DNA replication in salivary gland nuclei of Drosophila melanogaster at successive larval and prepupal stages. Genetics. 1967. Vol. 55. P. 375–386.

Atramentova L.О., Utevska O.M. Statistika dlia biologiv. Kharkiv: NTMT, 2014. 331 p. [in Ukrainian]

Marguerat S., Bähler J. Coordinating genome expression with cell size. Trends Genet. 2012. Vol. 28. P. 560–565. doi: 10.1016/j.tig.2012.07.003.

Fox D.T., Duronio R.J. Endoreplication and polyploidy: insights into development and disease. Development. 2013. Vol. 140. P. 3–12. doi: 10.1242/dev.080531.

Skorobagatko D.A., Mazilov A.A., Strashnyuk V.Yu. Endoreduplication in Drosophila melanogaster progeny after exposure to acute γ-irradiation. Radiat Environ Biophys. 2020. doi: 10.1007/s00411-019-00828-8.

Zhuravleva L.A., Strashnyuk V.Yu., Shakhbazov V.G. The influence of culture density on the polyteny degree of giant chromosomes in inbred lines and hybrids of Drosophila melanogaster. Tsitol Genet. 2004. Vol. 38. P. 46–51 [In Russian]

Dyka L.D., Shakina L.A., Strashnyuk V.Yu., Shckorbatov Yu.G. Effects of 36,6 GHz and static magnetic field on degree of endoreduplication in Drosophila melanogaster polytene chromosomes. Int. J. Radiat. Biol. 2016. Vol. 92. P. 222–227. doi: 10.3109/09553002.2016.1137105.

Øvrebø J.I., Edgar B.A. Polyploidy in tissue homeostasis and regeneration. Development. 2018. doi: 10.1242/dev.156034.

Sugimoto-Shirasu K., Roberts K. “Big it up”: endoreduplication and cell-size control in plants. Curr. Opin. Plant Biol. 2003. Vol. 6. P. 544–553. doi: 10.1016/j.pbi.2003.09.009

Shakina L.A., Strashnyuk V.Yu. Genetic, molecular, and humoral endocycle-regulating mechanisms. Rus. Journ. Genet. 2011. Vol. 47. P. 1151–1160. doi:10.1134/S1022795411100164.

Nesterkina M., Bilokon S., Alieksieieva T., Chubyk I., Kravchenko I. The influence of monoterpenoids and phenol deriva-tives on Drosophila melanogaster viability. Journal of Asia-Pacific Entomology. 2018. Vol. 21. P. 793–796. doi.org/10.1016/j.aspen.2018.06.004.

Bilokon S.V., Alieksieieva T.G. Stupin’ politenii gigantskykh chromosom Drosophila melanogaster za dii pestytsydiv. Drosophila v Experimentalnij Genetytsi ta Biologii: Materialy VI Mizhnarodnoi Konferentsii (Kharkiv, Serpen’ 20–24, 2018). Kharkiv, 2018. S. 12–15. [in Ukrainian]

Strashnyuk V.Yu., Taglina O.V., Bilokon S.V., Alieksieieva T.G. Metod vyznachennia toxychnoi dii khimichnoi rechovyny: pat. 116640 Ukraina: (2006.01) G01N 33/554. No. u 2019 03850; appl. 15.04.2019; publ. 11.11.2019, Bul. No. 21. 4 p. [in Ukrainian]