Marker-assisted selection for the gene of β-carotene hydroxylase in maize

  • O. V. Zatyshniak
  • V. Yu. Cherchel
  • B. V. Dziubetskyi
  • Jumei Zhang
  • Hui Jin
  • Rui Zhang
  • K. V. Denysiuk
  • T. M. Satarova
DOI: https://doi.org/10.7124/FEEO.v27.1307

Abstract

Aim. Estimation of the allelic status of  marker crtRB1-3'TE of  the β-carotene hydroxylase gene and marker-assisted selection by this marker in Ukrainian  maize breeding material. Methods. Field method and polymerase chain reaction. Results. The analysis of the allelic state of β-carotene hydroxylase gene for marker crtRB1-3'TE in maize breeding populations (DK23×F2)F2 and (DK23×F2)F3MAS having been obtained after the first and second self-pollinations of single cross DK23×F2 was provided. It was established that the parental inbred lines DK23 and F2 contained respectively 296 bp (unfavorable) and 543 bp (favorable) alleles of  marker crtRB1-3'TE. The three kinds of genotypes appeared to present at different frequencies in (DK23×F2)F2 – homozygous  for allele 296 bp, homozygous for allele 543 bp and heterozygous with both alleles 296 bp and 543 bp. For further cultivation and self-pollination, only plants with allele 543 bp within (DK23×F2)F2 were selected. All tested plants in population (DK23×F2)F3MAS were homozygous for allele 543 bp. Conclusions. Marker-associated selection in two generations for the β-carotene hydroxylase gene, involved in β-carotene accumulation, allowed to select homozygous plants of maize by favorable crtRB1-3'TE allele.

Keywords: Zea mays L., molecular genetic markers, carotenoids, breeding populations, allele.

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